Detailed information of TA system TA257080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 96679..97214
• Replicon: plasmid pWSM4643_2
• Accession: NZ_CP104042
• Organism: Rhizobium leguminosarum bv. viciae strain WSM4643

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A7Y2R4D4
• Locus tag: N1937_RS27330
• Protein ID: WP_017969175.1
• Coordinates: 96924..97214 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: N1937_RS27325
• Protein ID: WP_017969174.1
• Coordinates: 96679..96927 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N1937_RS27310 (N1937_27310)	92261..93970	+	1710	WP_260060100.1	ABC transporter ATP-binding protein	-
N1937_RS27315 (N1937_27315)	93990..95504	+	1515	WP_222386008.1	ABC transporter substrate-binding protein	-
N1937_RS27320 (N1937_27320)	95589..96464	-	876	WP_260060102.1	glutathione-dependent disulfide-bond oxidoreductase	-
N1937_RS27325 (N1937_27325)	96679..96927	+	249	WP_017969174.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
N1937_RS27330 (N1937_27330)	96924..97214	+	291	WP_017969175.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N1937_RS27335 (N1937_27335)	97226..98077	-	852	WP_260060105.1	NAD(P)-dependent oxidoreductase	-
N1937_RS27340 (N1937_27340)	98277..98744	+	468	WP_260060107.1	universal stress protein	-
N1937_RS27345 (N1937_27345)	98862..99776	+	915	WP_260060109.1	J domain-containing protein	-
N1937_RS27350 (N1937_27350)	99779..100102	+	324	WP_260060111.1	chaperone modulator CbpM	-
N1937_RS27355 (N1937_27355)	100117..101046	-	930	WP_260060113.1	phosphatidate cytidylyltransferase	-
N1937_RS27360 (N1937_27360)	101043..101663	-	621	WP_260060114.1	lysophospholipid acyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..548967	548967

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Antitoxin ParD (1-80)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11103.54 Da
• Isoelectric Point: 6.7147

>T257080 WP_017969175.1 NZ_CP104042:96924-97214 [Rhizobium leguminosarum bv. viciae]
MTDIIFSPAAQTDIDKIWDYTATSWNVDQAERYIQDIRDACQDLAEGTRMSRPSDIRKGYRKVSVGSHFLYFQSNDAGQI
IIVRILHQRMDVAKHL

Nucleotide

• Length: 291 bp

>T257080 NZ_CP104042:96924-97214 [Rhizobium leguminosarum bv. viciae]
ATGACCGACATCATATTTTCTCCCGCTGCACAAACCGATATTGACAAGATTTGGGATTACACAGCGACGAGTTGGAATGT
CGATCAGGCAGAGCGTTATATTCAGGACATAAGAGACGCCTGTCAGGATCTGGCGGAGGGAACACGGATGAGCCGCCCCT
CAGATATTCGGAAAGGGTACAGAAAGGTCTCCGTTGGCTCTCATTTTCTGTATTTCCAAAGCAATGATGCGGGCCAGATC
ATCATCGTGCGCATCCTTCATCAACGAATGGACGTAGCCAAGCACCTGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8970.05 Da
• Isoelectric Point: 6.4652

>AT257080 WP_017969174.1 NZ_CP104042:96679-96927 [Rhizobium leguminosarum bv. viciae]
MARNTSISLGEHFTTFIDTQVQAGRYGSATDVVRAGLRLLEEHEAKVKALQDALIEGEESGPATPFDFDAFNARKRAAFK
AK

Nucleotide

• Length: 249 bp

>AT257080 NZ_CP104042:96679-96927 [Rhizobium leguminosarum bv. viciae]
ATGGCCAGAAATACGTCCATATCGCTCGGCGAGCATTTCACCACCTTCATCGATACGCAAGTTCAGGCTGGCCGCTATGG
TTCAGCCACTGACGTTGTTCGGGCCGGCCTTCGCCTCCTTGAAGAACACGAAGCCAAAGTGAAGGCATTACAGGATGCCT
TGATTGAAGGCGAGGAATCCGGCCCTGCCACGCCCTTTGATTTCGATGCGTTCAACGCTCGCAAACGGGCGGCGTTCAAG
GCGAAATGA

Structures

Toxin

• AlphaFold DB: A0A7Y2R4D4