Detailed information of TA system TA025702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2200542..2201083
• Replicon: chromosome
• Accession: NC_016147
• Organism: Pseudoxanthomonas spadix BD-a59

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G7UPP8
• Locus tag: DSC_RS09925
• Protein ID: WP_014161034.1
• Coordinates: 2200542..2200868 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DSC_RS09930
• Protein ID: WP_014161035.1
• Coordinates: 2200865..2201083 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSC_RS09905	2196290..2196763	-	474	WP_014161031.1	GFA family protein	-
DSC_RS09910	2196772..2199336	-	2565	WP_043291218.1	ribonuclease R	-
DSC_RS09920	2199581..2200286	-	706	Protein_2006	transposase	-
DSC_RS09925	2200542..2200868	-	327	WP_014161034.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DSC_RS09930	2200865..2201083	-	219	WP_014161035.1	antitoxin MazE family protein	Antitoxin
DSC_RS09940	2201498..2203327	+	1830	WP_014161036.1	SLC13 family permease	-
DSC_RS09945	2203527..2205773	+	2247	WP_014161037.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11862.10 Da
• Isoelectric Point: 10.3164

>T25702 WP_014161034.1 NC_016147:c2200868-2200542 [Pseudoxanthomonas spadix BD-a59]
MKRGDFVTITMQGDFGKPRPALVIQANHFSESATVTVLPVTSTLVAAPLLRIKLQPEMENGLQKPSQVMIDKIMTVKREK
VGPTFGRLDANVLVEVERCLALFLGIAK

Nucleotide

• Length: 327 bp

>T25702 NC_016147:c2200868-2200542 [Pseudoxanthomonas spadix BD-a59]
ATGAAGCGCGGCGATTTTGTGACGATCACCATGCAGGGCGATTTCGGCAAGCCGCGCCCGGCGTTGGTGATCCAGGCCAA
CCACTTCAGCGAGAGCGCGACCGTGACGGTGTTGCCGGTGACCAGCACGTTGGTGGCTGCGCCACTGCTGCGGATCAAGC
TCCAGCCGGAAATGGAGAACGGCTTGCAGAAGCCGTCGCAGGTGATGATCGACAAGATCATGACGGTCAAGCGCGAGAAG
GTAGGGCCGACCTTCGGGCGATTGGATGCGAACGTGTTGGTCGAGGTGGAACGGTGCCTGGCGCTGTTCCTCGGTATCGC
GAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.21 Da
• Isoelectric Point: 5.5648

>AT25702 WP_014161035.1 NC_016147:c2201083-2200865 [Pseudoxanthomonas spadix BD-a59]
MAHVNSRVQKHRDALRVAGLRPVQIWVPDTRRPDFEQECHRQARLVAQADQADAGLQQLLDEAMAEVDGWTE

Nucleotide

• Length: 219 bp

>AT25702 NC_016147:c2201083-2200865 [Pseudoxanthomonas spadix BD-a59]
ATGGCCCATGTAAATTCCCGTGTCCAGAAGCATCGCGACGCCCTGCGCGTGGCGGGGTTGCGCCCGGTGCAGATCTGGGT
GCCCGATACCCGGCGACCGGACTTCGAGCAGGAGTGCCATCGCCAAGCGCGGTTGGTGGCCCAGGCTGACCAAGCCGATG
CCGGCCTGCAGCAGTTGCTGGATGAAGCGATGGCCGAGGTGGACGGCTGGACTGAATGA

Structures

Toxin

• AlphaFold DB: G7UPP8