Detailed information of TA system TA025701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1686380..1687164
• Replicon: chromosome
• Accession: NC_016147
• Organism: Pseudoxanthomonas spadix BD-a59

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: DSC_RS07505
• Protein ID: WP_043290471.1
• Coordinates: 1686380..1686877 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A3M6PNN3
• Locus tag: DSC_RS07510
• Protein ID: WP_043290472.1
• Coordinates: 1686874..1687164 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSC_RS07475	1682581..1683789	-	1209	WP_014160547.1	MFS transporter	-
DSC_RS16030	1684459..1684768	-	310	Protein_1516	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
DSC_RS07485	1685052..1685384	-	333	WP_014160549.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DSC_RS07490	1685381..1685632	-	252	WP_043290469.1	addiction module antitoxin	-
DSC_RS07495	1685676..1685924	-	249	WP_014160551.1	type II toxin-antitoxin system ParD family antitoxin	-
DSC_RS07500	1686103..1686387	+	285	WP_014160552.1	hypothetical protein	-
DSC_RS07505	1686380..1686877	-	498	WP_043290471.1	GNAT family N-acetyltransferase	Toxin
DSC_RS07510	1686874..1687164	-	291	WP_043290472.1	DUF1778 domain-containing protein	Antitoxin
DSC_RS07515	1687358..1687567	-	210	WP_014160555.1	hypothetical protein	-
DSC_RS07520	1688301..1690787	+	2487	WP_014160556.1	S8 family serine peptidase	-
DSC_RS07525	1690894..1691388	-	495	WP_014160557.1	SsrA-binding protein SmpB	-
DSC_RS07530	1691458..1691886	+	429	WP_014160558.1	type II toxin-antitoxin system RatA family toxin	-
DSC_RS07535	1691893..1692156	+	264	WP_014160559.1	RnfH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1670013..1687567	17554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18044.74 Da
• Isoelectric Point: 9.7537

>T25701 WP_043290471.1 NC_016147:c1686877-1686380 [Pseudoxanthomonas spadix BD-a59]
VSLSGAEPLGAQHRLEGFDCGKPTLNDWLVRHAPQAQGSGSAKTFVVSEDNRVAGYFSLTVGQIDTLDAPERIRKGLGQY
PIPVVILARLAVSLQDQGRGIGVGLLQDAIRRTLLIAEQAGIRAMLSHPIDEDAARFHTRFGFIGSPLREQQLLLLLKDA
RRWVK

Nucleotide

• Length: 498 bp

>T25701 NC_016147:c1686877-1686380 [Pseudoxanthomonas spadix BD-a59]
GTGAGCTTGAGCGGCGCAGAGCCGCTCGGGGCACAGCACCGGCTCGAAGGCTTCGATTGCGGCAAACCGACACTCAATGA
CTGGCTGGTGCGCCATGCCCCACAGGCGCAAGGCAGCGGCTCGGCCAAGACCTTCGTCGTCAGCGAAGACAACCGCGTCG
CTGGTTATTTCAGCCTGACCGTCGGCCAGATCGATACGCTCGATGCGCCGGAGCGCATTCGCAAGGGCCTGGGACAGTAT
CCGATCCCCGTCGTGATCCTTGCCCGTCTGGCCGTCAGCCTGCAGGATCAGGGCCGAGGGATCGGCGTCGGACTGTTGCA
GGATGCCATCCGCCGCACATTGCTGATTGCCGAGCAGGCCGGTATCCGCGCCATGCTGAGCCACCCCATCGACGAAGATG
CGGCACGGTTCCACACCCGCTTTGGTTTCATCGGCTCGCCGCTGCGTGAGCAGCAACTCTTACTGCTCCTCAAGGACGCC
CGCCGCTGGGTCAAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10566.90 Da
• Isoelectric Point: 5.0773

>AT25701 WP_043290472.1 NC_016147:c1687164-1686874 [Pseudoxanthomonas spadix BD-a59]
MAASTSSATRSARLGLRATPEQEAVLRRAADVAHKSLTDFILDSACQAAEQTLLDQRLFMVSGSQYQALMDLLEQPEQAN
EGLRDLFSRKAPWDKA

Nucleotide

• Length: 291 bp

>AT25701 NC_016147:c1687164-1686874 [Pseudoxanthomonas spadix BD-a59]
ATGGCTGCCTCTACATCTTCAGCGACTCGCTCCGCCCGTCTGGGATTGCGTGCCACGCCCGAGCAGGAAGCTGTTCTGCG
CCGCGCCGCCGATGTGGCGCACAAGTCGCTGACGGATTTCATCCTCGACAGCGCCTGCCAGGCCGCCGAGCAAACCCTGC
TCGACCAACGCTTGTTCATGGTCTCGGGAAGCCAGTACCAGGCCCTGATGGATTTGCTGGAACAGCCCGAACAGGCGAAC
GAAGGGCTGCGCGACCTGTTCTCACGGAAAGCCCCTTGGGACAAGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3M6PNN3