Detailed information of TA system TA025699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 679244..680706
• Replicon: chromosome
• Accession: NC_016147
• Organism: Pseudoxanthomonas spadix BD-a59

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: G7UNW7
• Locus tag: DSC_RS03000
• Protein ID: WP_014159645.1
• Coordinates: 679858..680706 (+)
• Length: 283 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: DSC_RS02995
• Protein ID: WP_043290362.1
• Coordinates: 679244..679861 (+)
• Length: 206 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSC_RS02970	674972..675313	-	342	WP_014159639.1	hypothetical protein	-
DSC_RS02975	675310..676710	-	1401	WP_014159640.1	integrating conjugative element protein	-
DSC_RS02980	676719..677654	-	936	WP_014159641.1	TIGR03756 family integrating conjugative element protein	-
DSC_RS02985	677651..678091	-	441	WP_014159642.1	TIGR03757 family integrating conjugative element protein	-
DSC_RS02990	678235..679005	-	771	WP_014159643.1	thioredoxin domain-containing protein	-
DSC_RS02995	679244..679861	+	618	WP_043290362.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
DSC_RS03000	679858..680706	+	849	WP_014159645.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
DSC_RS03005	680729..683620	-	2892	WP_014159646.1	conjugative transfer ATPase	-
DSC_RS03010	683623..684054	-	432	WP_014159647.1	TIGR03751 family conjugal transfer lipoprotein	-
DSC_RS03015	684035..685477	-	1443	WP_014159648.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-260)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (16-58)

Sequences

Toxin

Protein

• Length: 283 a.a.
• Molecular weight: 31577.27 Da
• Isoelectric Point: 6.8794

>T25699 WP_014159645.1 NC_016147:679858-680706 [Pseudoxanthomonas spadix BD-a59]
VSRTNLPASIRARLKQRADATRQDFNLTLTHYGLERLLYRLSISQYADRYLLKGALLFSLWYDQPHRPTRDVDLLGYGPD
DVETAVATFREISQIAVDDGIVFDPASVAGSVIRKEAGYGGVRVDLRATLDGARIALQVDIGFGDVVTPAPEPIHYPVLL
DDLPAPRLRAYPKYTVVAEKLHAICLLGMTNSRMKDYFDLWVLLTEEDLAPDELRRAVEATFARRRLAMPADLPIGLSDA
FAQDPIKQRQWAAFFKKNGLAPRELAEVVARLRDVFLALQSA

Nucleotide

• Length: 849 bp

>T25699 NC_016147:679858-680706 [Pseudoxanthomonas spadix BD-a59]
GTGAGCCGAACCAATCTACCCGCTTCGATCCGTGCCCGCCTGAAACAGCGCGCGGACGCGACGCGCCAGGACTTCAACCT
GACGCTGACCCATTACGGCCTGGAACGGCTGCTGTACCGGCTGTCGATCTCCCAATATGCCGATCGCTACCTGCTCAAGG
GCGCGCTGCTGTTTTCGCTGTGGTACGACCAGCCGCATCGGCCGACGCGCGATGTGGACCTGCTCGGCTATGGGCCGGAC
GACGTGGAGACGGCCGTGGCCACGTTCCGCGAGATCAGCCAGATCGCGGTGGACGACGGCATCGTGTTCGACCCCGCTTC
GGTCGCGGGGTCGGTGATTCGCAAGGAAGCCGGCTACGGAGGCGTGCGGGTCGATCTGCGGGCGACACTGGACGGTGCGC
GCATCGCCTTGCAGGTGGACATCGGTTTCGGCGATGTGGTGACGCCGGCGCCGGAGCCGATCCACTATCCGGTGCTGCTC
GACGATCTGCCCGCACCACGACTGCGCGCCTATCCCAAGTACACGGTGGTTGCGGAGAAGCTCCATGCCATCTGCCTGCT
GGGCATGACGAACAGCCGCATGAAGGACTACTTCGACCTGTGGGTGCTGCTGACCGAGGAGGACCTGGCGCCGGACGAAC
TGCGTCGAGCGGTCGAGGCGACCTTCGCGCGGCGGCGGCTGGCGATGCCGGCCGACCTTCCCATTGGCCTGAGCGACGCT
TTCGCGCAGGATCCGATCAAGCAGCGACAGTGGGCGGCGTTCTTCAAGAAGAACGGACTGGCGCCGCGGGAACTGGCCGA
GGTCGTCGCACGGCTGCGCGATGTCTTCCTGGCGCTGCAGTCGGCGTGA

Antitoxin

Protein

• Length: 206 a.a.
• Molecular weight: 22171.79 Da
• Isoelectric Point: 10.0954

>AT25699 WP_043290362.1 NC_016147:679244-679861 [Pseudoxanthomonas spadix BD-a59]
MPQRPDMSLEDAALVLARRQPLLRARDLTQRGLSTVILGRLVAAGKLERVARGVYSLPGRGLSEHRSLAEAALRVPKGVV
CLLSALRVHGIGTQAPHEVWMAIPHNSPSPSLDQPALRVVRMSGAALTEGIAPVAIDGVSVPVFDPNKTVADCFKYRNKI
GLDVALEALHDGWSQGKLSMDALWRYATVDRVATVMRPYLESVTA

Nucleotide

• Length: 618 bp

>AT25699 NC_016147:679244-679861 [Pseudoxanthomonas spadix BD-a59]
ATGCCTCAACGACCAGACATGAGCCTCGAAGATGCCGCCCTCGTGCTGGCCAGGCGCCAGCCGCTGCTGCGCGCGCGCGA
TCTGACCCAGCGCGGCCTGTCGACGGTGATCCTTGGCCGGCTGGTGGCGGCCGGCAAGCTGGAACGGGTCGCACGCGGCG
TCTACAGCCTGCCGGGACGTGGCCTCAGCGAGCACCGCTCACTGGCCGAGGCGGCGCTGCGCGTGCCCAAGGGCGTGGTC
TGCCTGCTGTCGGCGCTGCGCGTGCATGGCATCGGCACGCAGGCGCCGCACGAGGTCTGGATGGCGATCCCGCACAACTC
GCCATCGCCGAGCCTGGACCAGCCGGCGCTGCGCGTGGTGCGCATGTCGGGCGCGGCGCTGACCGAAGGCATCGCGCCGG
TGGCGATCGATGGCGTATCGGTGCCCGTGTTCGATCCGAACAAGACCGTGGCCGACTGCTTCAAATACCGCAACAAGATC
GGGCTGGACGTGGCCCTGGAGGCGCTGCACGACGGGTGGTCGCAGGGCAAGCTGTCGATGGATGCACTCTGGCGCTACGC
GACGGTGGATCGCGTGGCCACGGTGATGCGCCCCTATCTGGAAAGCGTGACCGCGTGA

Structures

Toxin

• AlphaFold DB: G7UNW7