Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 519591..520227 Replicon chromosome
Accession NZ_CP103995
Organism Bacillus subtilis strain SRCM117109

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag N0B18_RS02650 Protein ID WP_003156187.1
Coordinates 519877..520227 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag N0B18_RS02645 Protein ID WP_003225183.1
Coordinates 519591..519872 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0B18_RS02625 (N0B18_02625) 515950..516549 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
N0B18_RS02630 (N0B18_02630) 516644..517009 + 366 WP_069963989.1 holo-ACP synthase -
N0B18_RS02635 (N0B18_02635) 517175..518191 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
N0B18_RS02640 (N0B18_02640) 518306..519475 + 1170 WP_015252766.1 alanine racemase -
N0B18_RS02645 (N0B18_02645) 519591..519872 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
N0B18_RS02650 (N0B18_02650) 519877..520227 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
N0B18_RS02655 (N0B18_02655) 520343..521167 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
N0B18_RS02660 (N0B18_02660) 521172..521537 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
N0B18_RS02665 (N0B18_02665) 521541..521942 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
N0B18_RS02670 (N0B18_02670) 521954..522961 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
N0B18_RS02675 (N0B18_02675) 523023..523352 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
N0B18_RS02680 (N0B18_02680) 523349..523831 + 483 WP_003234299.1 anti-sigma B factor RsbW -
N0B18_RS02685 (N0B18_02685) 523797..524585 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
N0B18_RS02690 (N0B18_02690) 524585..525184 + 600 WP_021481701.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T256964 WP_003156187.1 NZ_CP103995:519877-520227 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT256964 WP_003225183.1 NZ_CP103995:519591-519872 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References