Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4539539..4540036 Replicon chromosome
Accession NZ_CP103974
Organism Escherichia coli strain DLI.6n

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NZ657_RS22155 Protein ID WP_001173343.1
Coordinates 4539539..4539712 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NZ657_RS22160 Protein ID WP_000398619.1
Coordinates 4539740..4540036 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NZ657_RS22145 (4536209) 4536209..4537666 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
NZ657_RS22150 (4537903) 4537903..4539420 + 1518 WP_001295074.1 lysine--tRNA ligase -
NZ657_RS22155 (4539539) 4539539..4539712 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NZ657_RS22160 (4539740) 4539740..4540036 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NZ657_RS22165 (4540263) 4540263..4540535 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
NZ657_RS22170 (4540547) 4540547..4540777 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NZ657_RS22175 (4540958) 4540958..4542589 + 1632 WP_077487507.1 sensor histidine kinase -
NZ657_RS22180 (4542586) 4542586..4543305 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4515944..4540036 24092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T256953 WP_001173343.1 NZ_CP103974:c4539712-4539539 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT256953 WP_000398619.1 NZ_CP103974:c4540036-4539740 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References