Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3232956..3233453 Replicon chromosome
Accession NZ_CP103972
Organism Escherichia coli strain DLI.5a

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NZD79_RS15695 Protein ID WP_001173343.1
Coordinates 3233280..3233453 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NZD79_RS15690 Protein ID WP_000398619.1
Coordinates 3232956..3233252 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NZD79_RS15670 (3229687) 3229687..3230406 - 720 WP_000611288.1 two-component system response regulator DcuR -
NZD79_RS15675 (3230403) 3230403..3232034 - 1632 WP_001216477.1 sensor histidine kinase -
NZD79_RS15680 (3232215) 3232215..3232445 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NZD79_RS15685 (3232457) 3232457..3232729 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
NZD79_RS15690 (3232956) 3232956..3233252 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NZD79_RS15695 (3233280) 3233280..3233453 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NZD79_RS15700 (3233572) 3233572..3235089 - 1518 WP_001295074.1 lysine--tRNA ligase -
NZD79_RS15705 (3235326) 3235326..3236783 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T256931 WP_001173343.1 NZ_CP103972:3233280-3233453 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT256931 WP_000398619.1 NZ_CP103972:3232956-3233252 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References