Detailed information of TA system TA025693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3927848..3928410
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MEALZ_RS22285
• Protein ID: WP_084685518.1
• Coordinates: 3927848..3928165 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: G4SUC1
• Locus tag: MEALZ_RS16675
• Protein ID: WP_014149826.1
• Coordinates: 3928165..3928410 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS16655	3923202..3923873	-	672	WP_014149823.1	HAMP domain-containing histidine kinase	-
MEALZ_RS16660	3923873..3925519	-	1647	WP_014149824.1	response regulator	-
MEALZ_RS16665	3925615..3927534	+	1920	WP_014149825.1	molecular chaperone HtpG	-
MEALZ_RS22285	3927848..3928165	-	318	WP_084685518.1	CcdB family protein	Toxin
MEALZ_RS16675	3928165..3928410	-	246	WP_014149826.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MEALZ_RS16680	3928576..3929424	-	849	WP_014149827.1	polyamine aminopropyltransferase	-
MEALZ_RS16685	3929824..3931659	+	1836	WP_014149828.1	biosynthetic arginine decarboxylase	-
MEALZ_RS16690	3931674..3932540	+	867	WP_162473022.1	NAD(P)-dependent oxidoreductase	-
MEALZ_RS16695	3932772..3933068	+	297	WP_014149830.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-104)

Antitoxin

• Post-segregation antitoxin CcdA (11-81)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11648.73 Da
• Isoelectric Point: 8.2414

>T25693 WP_084685518.1 NC_016112:c3928165-3927848 [Methylotuvimicrobium alcaliphilum 20Z]
VAQFRAYENSNKATMKAYPYLLDIQSNLLDELRTTVVIPLCPTNLAGKAAITKLCPILKIDGRSFIVLTQQIAGIDRKYL
GKEICDLSLYRSEIIAALDFIISGI

Nucleotide

• Length: 318 bp

>T25693 NC_016112:c3928165-3927848 [Methylotuvimicrobium alcaliphilum 20Z]
GTGGCTCAGTTCAGGGCTTACGAGAATTCAAACAAAGCAACTATGAAAGCCTACCCTTACCTTTTGGATATCCAATCCAA
CTTACTTGACGAGTTACGGACCACAGTCGTCATTCCGCTTTGCCCTACTAACCTTGCAGGTAAAGCTGCAATTACAAAGC
TATGTCCAATATTAAAAATTGATGGCAGATCTTTTATTGTGCTCACCCAGCAAATTGCCGGCATAGATCGAAAATATCTT
GGTAAAGAAATTTGCGACCTTTCGCTGTATCGCTCTGAAATTATTGCCGCATTGGACTTCATCATTTCCGGCATTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9135.42 Da
• Isoelectric Point: 5.6566

>AT25693 WP_014149826.1 NC_016112:c3928410-3928165 [Methylotuvimicrobium alcaliphilum 20Z]
MPPVFDTHAPKKPTNVSINSDLLVKAKELKINLSATLETALTELVNARQRELWLCENKIAIEAYNQLVENHGNFSDDLRG
F

Nucleotide

• Length: 246 bp

>AT25693 NC_016112:c3928410-3928165 [Methylotuvimicrobium alcaliphilum 20Z]
ATGCCCCCGGTCTTTGACACCCATGCCCCAAAAAAACCGACAAACGTCAGTATAAACAGTGATTTACTTGTTAAAGCGAA
AGAGCTAAAGATCAATTTGTCGGCAACCTTGGAAACAGCTCTAACCGAATTAGTAAACGCTCGGCAACGAGAACTTTGGC
TGTGCGAAAACAAGATTGCCATCGAAGCGTACAATCAATTGGTAGAGAATCATGGAAACTTTAGCGACGATTTGAGGGGC
TTTTAG

Structures

Antitoxin

• AlphaFold DB: G4SUC1