Detailed information of TA system TA025692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-StbC
• Location: 3784600..3785242
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MEALZ_RS15935
• Protein ID: WP_014149687.1
• Coordinates: 3784823..3785242 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G4T4B7
• Locus tag: MEALZ_RS15930
• Protein ID: WP_014149686.1
• Coordinates: 3784600..3784836 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS15905	3780331..3781134	+	804	WP_014149681.1	class I SAM-dependent methyltransferase	-
MEALZ_RS15910	3781169..3782239	+	1071	WP_046061209.1	PD40 domain-containing protein	-
MEALZ_RS15915	3782535..3782873	-	339	WP_014149683.1	hypothetical protein	-
MEALZ_RS15920	3783183..3783500	-	318	WP_014149684.1	BrnA antitoxin family protein	-
MEALZ_RS23095	3783504..3783647	-	144	Protein_3111	BrnT family toxin	-
MEALZ_RS15925	3783678..3784232	-	555	WP_014149685.1	Uma2 family endonuclease	-
MEALZ_RS15930	3784600..3784836	+	237	WP_014149686.1	DNA-binding protein	Antitoxin
MEALZ_RS15935	3784823..3785242	+	420	WP_014149687.1	type II toxin-antitoxin system VapC family toxin	Toxin
MEALZ_RS15940	3785894..3786178	-	285	WP_017840667.1	DUF2442 domain-containing protein	-
MEALZ_RS15945	3786371..3787219	-	849	WP_014149689.1	ABC transporter permease	-
MEALZ_RS15950	3787319..3788041	-	723	WP_046061210.1	hypothetical protein	-
MEALZ_RS15955	3788548..3789168	-	621	WP_014149691.1	hypothetical protein	-
MEALZ_RS23070	3789184..3789888	-	705	WP_174888151.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15916.30 Da
• Isoelectric Point: 5.2145

>T25692 WP_014149687.1 NC_016112:3784823-3785242 [Methylotuvimicrobium alcaliphilum 20Z]
MYLIDTNIISELRKGYKANSGVQEFFDKVIQENVPIYISAITIGELRRGVDLIFHRGDETQGKLLENWLNSILDQYQDNI
LTIDSEIAMLWGKLRVPRHEHALDKLIAATGIIYGLVIVTRNTKDFENTGVRVLNPFIE

Nucleotide

• Length: 420 bp

>T25692 NC_016112:3784823-3785242 [Methylotuvimicrobium alcaliphilum 20Z]
ATGTATTTGATTGATACCAACATCATCAGCGAGCTTAGAAAAGGCTATAAAGCCAATTCCGGTGTGCAAGAATTTTTCGA
TAAAGTCATTCAGGAGAATGTCCCCATTTACATTTCCGCCATTACGATTGGCGAATTGCGCAGAGGCGTCGATTTGATTT
TCCACCGTGGCGACGAAACACAAGGCAAACTGTTGGAAAACTGGCTCAATAGCATCCTCGATCAATATCAAGACAACATC
CTGACCATTGATAGCGAAATTGCTATGCTTTGGGGAAAATTACGCGTCCCTAGGCACGAACATGCCTTAGATAAATTGAT
TGCAGCTACCGGGATAATCTATGGTCTAGTCATTGTTACTAGAAACACCAAAGACTTCGAAAATACTGGTGTTCGAGTGT
TAAATCCTTTTATTGAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8744.82 Da
• Isoelectric Point: 5.5882

>AT25692 WP_014149686.1 NC_016112:3784600-3784836 [Methylotuvimicrobium alcaliphilum 20Z]
MPSLVVRNLDDSIINALKERAVKHHRSTEAEHRAILAEVLMKPQRKSFAEALANIPAVGTDTDFQRINDDTSESNVFD

Nucleotide

• Length: 237 bp

>AT25692 NC_016112:3784600-3784836 [Methylotuvimicrobium alcaliphilum 20Z]
ATGCCAAGTCTAGTCGTTAGAAACCTTGATGATTCAATCATAAATGCGTTGAAAGAAAGAGCGGTTAAACACCATCGCTC
TACTGAAGCCGAGCATCGAGCCATTTTGGCTGAAGTCCTGATGAAACCTCAACGTAAAAGCTTTGCTGAAGCTTTAGCCA
ACATACCTGCTGTTGGCACGGATACGGATTTTCAGCGCATCAATGACGATACTAGCGAATCCAATGTATTTGATTGA

Structures

Antitoxin

• AlphaFold DB: G4T4B7