Detailed information of TA system TA025691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 3253400..3254199
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: -
• Uniprot ID: G4T0B3
• Locus tag: MEALZ_RS13790
• Protein ID: WP_014149269.1
• Coordinates: 3253400..3253933 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: G4T0B4
• Locus tag: MEALZ_RS13795
• Protein ID: WP_014149270.1
• Coordinates: 3253930..3254199 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS22115	3249330..3249539	-	210	WP_014149265.1	addiction module protein	-
MEALZ_RS13775	3249836..3250054	-	219	WP_014149266.1	type II toxin-antitoxin system HicB family antitoxin	-
MEALZ_RS13780	3250331..3251557	-	1227	WP_014149267.1	restriction endonuclease subunit S	-
MEALZ_RS13785	3251554..3253056	-	1503	WP_014149268.1	N-6 DNA methylase	-
MEALZ_RS13790	3253400..3253933	-	534	WP_014149269.1	GNAT family N-acetyltransferase	Toxin
MEALZ_RS13795	3253930..3254199	-	270	WP_014149270.1	DUF1778 domain-containing protein	Antitoxin
MEALZ_RS13800	3255097..3255765	-	669	WP_014149271.1	hypothetical protein	-
MEALZ_RS13805	3256035..3256214	+	180	WP_014149272.1	helix-turn-helix domain-containing protein	-
MEALZ_RS13825	3256806..3257003	-	198	WP_014149273.1	carbon storage regulator CsrA	-
MEALZ_RS13830	3257096..3258325	-	1230	WP_014149274.1	aspartate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-81)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19952.33 Da
• Isoelectric Point: 9.4278

>T25691 WP_014149269.1 NC_016112:c3253933-3253400 [Methylotuvimicrobium alcaliphilum 20Z]
VSWGKEFLELSKSKHDRNSFDCGVQELNTFIKTQAARHMEAGISRTMVLPSAHPLLNQQFAICAFYSVAPSSISRETLPA
QLAKKLPKYPVPVFLLAQLAVHKEFHGAGLGKVCLIRALKYLWDVNHYMRAYAIVVDCLTDSAQAFYAKFGFEVLCGHNG
RIRMFLPMKTVEQLFSQ

Nucleotide

• Length: 534 bp

>T25691 NC_016112:c3253933-3253400 [Methylotuvimicrobium alcaliphilum 20Z]
GTGAGTTGGGGCAAAGAGTTCCTAGAACTCAGTAAATCAAAACATGATCGCAACTCGTTTGATTGTGGCGTGCAAGAGCT
AAACACGTTTATCAAAACTCAAGCAGCAAGGCATATGGAAGCGGGGATCAGCCGCACAATGGTTTTGCCAAGTGCCCACC
CATTATTAAACCAACAGTTTGCCATTTGCGCATTTTATAGCGTTGCTCCAAGTTCAATTAGTCGTGAGACGCTGCCGGCA
CAACTTGCTAAAAAACTTCCTAAATATCCGGTACCTGTTTTCTTGCTTGCACAATTAGCGGTGCATAAAGAGTTTCATGG
TGCGGGACTTGGTAAAGTTTGCCTTATTCGCGCGTTGAAATACTTGTGGGATGTGAACCATTACATGAGAGCCTATGCAA
TTGTTGTCGACTGCTTGACCGATTCAGCACAAGCCTTTTATGCGAAGTTCGGCTTCGAGGTTTTGTGTGGACACAATGGA
CGCATCCGTATGTTCTTACCGATGAAAACGGTAGAACAGCTTTTCAGCCAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9815.19 Da
• Isoelectric Point: 4.7341

>AT25691 WP_014149270.1 NC_016112:c3254199-3253930 [Methylotuvimicrobium alcaliphilum 20Z]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLINEDATHVIEKHESITVRDSVFDEFIAACDKAKAPNRALLDAA
KFAEESGIE

Nucleotide

• Length: 270 bp

>AT25691 NC_016112:c3254199-3253930 [Methylotuvimicrobium alcaliphilum 20Z]
ATGGCTACAGCTCGTTTAGATATTCGACTTGACGAAGAAATCAAGGCTAAAGCGGAAAAAGCTTCAGCTTTGCTTGGGTT
AAAAAGCCTAACAGAGTACGTTGTTAGGCTAATAAACGAAGACGCAACTCATGTTATAGAAAAACATGAAAGTATTACAG
TCAGAGACAGTGTGTTTGATGAATTTATTGCAGCGTGTGATAAAGCAAAAGCGCCCAACCGAGCTCTACTAGATGCTGCT
AAATTTGCAGAAGAGAGCGGCATTGAGTGA

Structures

Toxin

• AlphaFold DB: G4T0B3

Antitoxin

• AlphaFold DB: G4T0B4