Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 2201882..2203019 Replicon chromosome
Accession NZ_CP103964
Organism Staphylococcus hyicus strain MM2101

Toxin (Protein)


Gene name zeta Uniprot ID D1MAU9
Locus tag NZD48_RS10765 Protein ID WP_002333464.1
Coordinates 2201882..2202745 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag NZD48_RS10770 Protein ID WP_000301765.1
Coordinates 2202747..2203019 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NZD48_RS10740 (NZD48_10740) 2196895..2197740 - 846 WP_000733283.1 BlaZ family penicillin-hydrolyzing class A beta-lactamase PC1 -
NZD48_RS10745 (NZD48_10745) 2197847..2199604 + 1758 WP_001096374.1 beta-lactam sensor/signal transducer BlaR1 -
NZD48_RS10750 (NZD48_10750) 2199594..2199974 + 381 WP_001284656.1 penicillinase repressor BlaI -
NZD48_RS10755 (NZD48_10755) 2200238..2200831 + 594 WP_000690636.1 recombinase family protein -
NZD48_RS10760 (NZD48_10760) 2201070..2201441 - 372 WP_259489101.1 hypothetical protein -
NZD48_RS10765 (NZD48_10765) 2201882..2202745 - 864 WP_002333464.1 zeta toxin family protein Toxin
NZD48_RS10770 (NZD48_10770) 2202747..2203019 - 273 WP_000301765.1 antitoxin Antitoxin
NZD48_RS10775 (NZD48_10775) 2203036..2203251 - 216 WP_002321610.1 peptide-binding protein -
NZD48_RS10780 (NZD48_10780) 2203322..2204218 - 897 WP_001809760.1 ParA family protein -
NZD48_RS10785 (NZD48_10785) 2204286..2204546 - 261 Protein_2096 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
NZD48_RS10790 (NZD48_10790) 2204716..2205453 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
NZD48_RS10795 (NZD48_10795) 2205578..2205673 - 96 WP_001809736.1 23S rRNA methyltransferase attenuation leader peptide -
NZD48_RS10800 (NZD48_10800) 2205742..2205864 - 123 Protein_2099 peptide-binding protein -
NZD48_RS10805 (NZD48_10805) 2205984..2207510 - 1527 WP_259489110.1 type IA DNA topoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island blaZ / erm(B) / ant(6)-Ia - 2190145..2216715 26570
- inside IScluster/Tn erm(B) / ant(6)-Ia - 2204716..2216715 11999


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32370.87 Da        Isoelectric Point: 6.6610

>T256909 WP_002333464.1 NZ_CP103964:c2202745-2201882 [Staphylococcus hyicus]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT256909 WP_000301765.1 NZ_CP103964:c2203019-2202747 [Staphylococcus hyicus]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D1MAU9

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References