Detailed information of TA system TA025690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 3216138..3216769
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G4SZP3
• Locus tag: MEALZ_RS13670
• Protein ID: WP_014149243.1
• Coordinates: 3216138..3216524 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G4SZP4
• Locus tag: MEALZ_RS13675
• Protein ID: WP_014149244.1
• Coordinates: 3216521..3216769 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS13650	3214370..3214624	-	255	WP_014149239.1	hypothetical protein	-
MEALZ_RS13655	3214621..3214821	-	201	WP_014149240.1	hypothetical protein	-
MEALZ_RS22100	3215021..3215164	-	144	Protein_2674	PD-(D/E)XK nuclease family transposase	-
MEALZ_RS13665	3215786..3216010	-	225	WP_014149242.1	addiction module protein	-
MEALZ_RS13670	3216138..3216524	-	387	WP_014149243.1	type II toxin-antitoxin system VapC family toxin	Toxin
MEALZ_RS13675	3216521..3216769	-	249	WP_014149244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MEALZ_RS22780	3217214..3217354	+	141	WP_014149246.1	hypothetical protein	-
MEALZ_RS13680	3217351..3217701	-	351	WP_014149247.1	DUF86 domain-containing protein	-
MEALZ_RS13685	3217691..3218002	-	312	WP_014149248.1	nucleotidyltransferase	-
MEALZ_RS13695	3218311..3219162	-	852	Protein_2681	PD-(D/E)XK nuclease family transposase	-
MEALZ_RS13700	3219889..3221724	+	1836	WP_014149250.1	S8 family serine peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (4-126)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-47)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14430.70 Da
• Isoelectric Point: 6.2149

>T25690 WP_014149243.1 NC_016112:c3216524-3216138 [Methylotuvimicrobium alcaliphilum 20Z]
MNLLLDTHVALWAITDNSKLSQKARDLIVSPKATVWVSAANVWEIAIKHSLGRGDMPVSGRDAMRYFQESGYRFLAVEAE
HTIAVEELPLHHQDPFDRILVAQALVEPMRLMTHDPLVALYSDTIIKI

Nucleotide

• Length: 387 bp

>T25690 NC_016112:c3216524-3216138 [Methylotuvimicrobium alcaliphilum 20Z]
TTGAATCTGCTGCTGGATACTCACGTTGCACTATGGGCTATCACGGATAACTCAAAACTGTCACAAAAGGCGCGTGATTT
GATTGTATCGCCCAAAGCAACCGTTTGGGTCAGCGCGGCGAATGTATGGGAAATTGCAATCAAGCACTCGCTAGGGCGAG
GCGACATGCCGGTTTCTGGCCGAGACGCGATGCGCTATTTTCAGGAATCCGGATATCGCTTCCTTGCTGTCGAGGCGGAA
CATACGATAGCGGTCGAAGAACTGCCTTTACATCACCAAGACCCGTTCGACCGCATACTTGTGGCTCAGGCGCTCGTAGA
GCCGATGCGTTTAATGACCCACGATCCCTTGGTAGCGCTCTATAGCGACACAATTATCAAGATTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8981.32 Da
• Isoelectric Point: 5.0506

>AT25690 WP_014149244.1 NC_016112:c3216769-3216521 [Methylotuvimicrobium alcaliphilum 20Z]
MQTVNMLQAKSSLSRLVEAIEQGQEREIVIARNGRPAAKLVPIDAAPSEKRIGVAKGLFVVPDSIDTYNDEVARLFMGEV
QN

Nucleotide

• Length: 249 bp

>AT25690 NC_016112:c3216769-3216521 [Methylotuvimicrobium alcaliphilum 20Z]
ATGCAAACTGTAAATATGTTACAAGCCAAGTCTTCTCTGTCCCGCTTAGTAGAAGCCATTGAGCAAGGGCAAGAGCGAGA
AATCGTGATTGCCAGGAATGGCCGCCCAGCTGCTAAGCTTGTTCCGATAGATGCTGCGCCATCCGAGAAGCGGATTGGAG
TAGCAAAAGGCTTGTTCGTGGTGCCGGACAGCATTGATACATATAACGACGAAGTGGCTCGGTTGTTCATGGGCGAGGTG
CAGAATTGA

Structures

Toxin

• AlphaFold DB: G4SZP3

Antitoxin

• AlphaFold DB: G4SZP4