Detailed information of TA system TA025689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2448016..2448496
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MEALZ_RS21890
• Protein ID: WP_198482361.1
• Coordinates: 2448275..2448496 (-)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: G4ST97
• Locus tag: MEALZ_RS10590
• Protein ID: WP_014148638.1
• Coordinates: 2448016..2448285 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS10580	2443082..2445538	-	2457	WP_046061528.1	CRISPR-associated helicase/endonuclease Cas3	-
MEALZ_RS10585	2445982..2447232	-	1251	WP_014148637.1	group II intron reverse transcriptase/maturase	-
MEALZ_RS10590	2448016..2448285	-	270	WP_014148638.1	hypothetical protein	Antitoxin
MEALZ_RS21890	2448275..2448496	-	222	WP_198482361.1	BrnT family toxin	Toxin
MEALZ_RS10605	2449109..2450434	-	1326	WP_014148639.1	ATP-binding protein	-
MEALZ_RS22690	2451061..2451260	+	200	Protein_2073	hypothetical protein	-
MEALZ_RS10610	2451344..2452585	+	1242	WP_014146701.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2443082..2489713	46631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (2-67)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8571.72 Da
• Isoelectric Point: 7.2033

>T25689 WP_198482361.1 NC_016112:c2448496-2448275 [Methylotuvimicrobium alcaliphilum 20Z]
MEKHGIDFLDAQALRRDTDLLELQARSDDEPRFLVIGLIDSKHWSAVVTYRNGTVRLISVRRSRKREVELYES

Nucleotide

• Length: 222 bp

>T25689 NC_016112:c2448496-2448275 [Methylotuvimicrobium alcaliphilum 20Z]
CTGGAAAAGCACGGAATCGACTTTCTGGATGCCCAGGCCCTCAGGCGAGACACTGACCTGCTGGAACTCCAAGCTAGATC
GGATGATGAACCTCGTTTTCTGGTCATAGGTCTGATTGATTCGAAACACTGGTCAGCCGTCGTCACCTATAGAAACGGAA
CAGTTCGGCTTATTTCTGTAAGGCGCTCCCGCAAACGAGAGGTAGAACTATATGAAAGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10092.47 Da
• Isoelectric Point: 9.8407

>AT25689 WP_014148638.1 NC_016112:c2448285-2448016 [Methylotuvimicrobium alcaliphilum 20Z]
MKAKDFDKKFDQGKKDIIDDLDLSTARRVNQEQKRINVDFPSWVVDSLDREAARIGVTRQLIIKVWLVERLKAEAANKAL
NGDASNGAH

Nucleotide

• Length: 270 bp

>AT25689 NC_016112:c2448285-2448016 [Methylotuvimicrobium alcaliphilum 20Z]
ATGAAAGCTAAAGATTTTGATAAGAAATTTGACCAAGGTAAAAAAGACATCATTGATGATCTTGACTTATCCACGGCGCG
TCGTGTCAACCAGGAACAGAAGCGCATAAATGTCGACTTTCCTTCGTGGGTAGTAGACTCGTTGGACCGTGAAGCGGCTC
GTATCGGTGTTACCCGGCAATTGATCATCAAGGTCTGGCTGGTTGAGCGCTTGAAAGCTGAAGCTGCTAACAAAGCCCTC
AATGGTGATGCCTCAAACGGCGCACATTAG

Structures

Antitoxin

• AlphaFold DB: G4ST97