Detailed information of TA system TA025688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2434105..2434670
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G4ST86
• Locus tag: MEALZ_RS10535
• Protein ID: WP_014148627.1
• Coordinates: 2434105..2434473 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G4ST87
• Locus tag: MEALZ_RS10540
• Protein ID: WP_014148628.1
• Coordinates: 2434476..2434670 (-)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS10520	2430350..2431819	+	1470	WP_014148623.1	efflux transporter outer membrane subunit	-
MEALZ_RS10525	2432063..2432671	-	609	WP_014148624.1	hypothetical protein	-
MEALZ_RS22685	2432924..2433094	-	171	WP_162472945.1	hypothetical protein	-
MEALZ_RS10530	2433087..2434094	-	1008	WP_046061527.1	virulence protein RhuM/Fic/DOC family protein	-
MEALZ_RS10535	2434105..2434473	-	369	WP_014148627.1	type II toxin-antitoxin system VapC family toxin	Toxin
MEALZ_RS10540	2434476..2434670	-	195	WP_014148628.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MEALZ_RS10550	2437452..2437742	-	291	WP_026130023.1	CRISPR-associated endonuclease Cas2	-
MEALZ_RS10555	2437757..2438791	-	1035	WP_014148631.1	type I-C CRISPR-associated endonuclease Cas1	-
MEALZ_RS10560	2438794..2439420	-	627	WP_014148632.1	CRISPR-associated protein Cas4	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13460.62 Da
• Isoelectric Point: 6.0660

>T25688 WP_014148627.1 NC_016112:c2434473-2434105 [Methylotuvimicrobium alcaliphilum 20Z]
MPVLVDTSVWIDHFRKKEPALVALLESAQVLGHPFVRGELALGNLPQRHTLLELLDNLPQTPVAFGEEINRFIEANTLFG
LGIGFVDVHLLASAKLALNATLWTRDNRLLAVATQLNVGYQP

Nucleotide

• Length: 369 bp

>T25688 NC_016112:c2434473-2434105 [Methylotuvimicrobium alcaliphilum 20Z]
ATGCCGGTATTAGTGGATACCTCGGTATGGATCGACCACTTTCGCAAAAAAGAACCGGCGTTAGTCGCTTTATTGGAAAG
CGCTCAAGTTTTAGGTCATCCGTTTGTCCGGGGTGAACTCGCGTTAGGTAATTTACCGCAAAGGCATACTCTTTTGGAGC
TATTGGATAACTTGCCGCAAACGCCGGTTGCGTTCGGTGAGGAAATCAACCGGTTTATTGAAGCGAATACCTTGTTTGGT
TTGGGCATCGGTTTTGTCGATGTGCATCTGTTAGCCTCTGCAAAATTGGCGCTTAACGCCACCTTATGGACACGCGATAA
CCGCCTACTGGCCGTTGCTACGCAGCTCAATGTGGGCTATCAACCCTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7111.31 Da
• Isoelectric Point: 8.7481

>AT25688 WP_014148628.1 NC_016112:c2434670-2434476 [Methylotuvimicrobium alcaliphilum 20Z]
MRTTVVLDDELLEKAQALTNLKEKSSLIKEALKALIERESAKRLASLGGIAPDLQPIPRRQTEL

Nucleotide

• Length: 195 bp

>AT25688 NC_016112:c2434670-2434476 [Methylotuvimicrobium alcaliphilum 20Z]
ATGCGCACGACAGTTGTATTAGATGATGAATTACTCGAAAAAGCTCAAGCCTTAACCAACCTAAAAGAAAAATCCAGCCT
CATCAAAGAAGCCTTGAAGGCCCTGATTGAACGCGAGAGCGCCAAGCGACTAGCTAGCTTAGGCGGTATTGCCCCTGATT
TGCAGCCCATCCCGCGCCGGCAAACCGAGTTGTAA

Structures

Toxin

• AlphaFold DB: G4ST86

Antitoxin

• AlphaFold DB: G4ST87