Detailed information of TA system TA256876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1652139..1653874
• Replicon: chromosome
• Accession: NZ_CP103872
• Organism: Herbaspirillum huttiense strain ZXN111

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: NY669_RS07245
• Protein ID: WP_259434528.1
• Coordinates: 1652139..1653035 (+)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: NY669_RS07250
• Protein ID: WP_166675535.1
• Coordinates: 1653041..1653874 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NY669_RS07220 (NY669_07220)	1647997..1648782	+	786	WP_039787822.1	ABC transporter ATP-binding protein	-
NY669_RS07225 (NY669_07225)	1648848..1649843	+	996	WP_034340420.1	ABC transporter substrate-binding protein	-
NY669_RS07230 (NY669_07230)	1649846..1650706	+	861	WP_259434526.1	ABC transporter permease	-
NY669_RS07235 (NY669_07235)	1650747..1651517	+	771	WP_259434527.1	SDR family oxidoreductase	-
NY669_RS07240 (NY669_07240)	1651585..1652121	+	537	WP_034340408.1	cupin domain-containing protein	-
NY669_RS07245 (NY669_07245)	1652139..1653035	+	897	WP_259434528.1	alpha/beta hydrolase	Toxin
NY669_RS07250 (NY669_07250)	1653041..1653874	+	834	WP_166675535.1	IclR family transcriptional regulator	Antitoxin
NY669_RS07255 (NY669_07255)	1653876..1654700	+	825	WP_034340424.1	SDR family oxidoreductase	-
NY669_RS07260 (NY669_07260)	1654693..1656414	+	1722	WP_209582984.1	thiamine pyrophosphate-binding protein	-
NY669_RS07265 (NY669_07265)	1656425..1657225	+	801	WP_259434529.1	aspartate dehydrogenase	-
NY669_RS07270 (NY669_07270)	1657248..1658726	+	1479	WP_259434530.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (53-285)

Antitoxin

• Transcription regulator IclR, N-terminal (20-68)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 31673.89 Da
• Isoelectric Point: 7.3885

>T256876 WP_259434528.1 NZ_CP103872:1652139-1653035 [Herbaspirillum huttiense]
MSAFLSTGSATSREALSASLALLDCHFPEQRVALPDGQVSYRSAGRSDRDQVVVLLHGISSGAASWLHMALALANDARVI
AWNAPGYGSSTALPMAMPRASDYAARLQQMLAALEISQCVLVGHSLGALMAAAYLARGDGRAARTLLADPALGYGGAGQQ
ERSVQVRQERLGALQRLGVAGLAAQRTRLLSTQASDEQHAWVRWNMQRLDVAGYTQAVHLLCGDDIATYLQAAPRRGLSV
ACGAEDIVTTPQASEGLARQFEIPFQLVDGAGHACYIEQPLSFAALVRAQFTSTNQNR

Nucleotide

• Length: 897 bp

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30909.39 Da
• Isoelectric Point: 6.3942

>AT256876 WP_166675535.1 NZ_CP103872:1653041-1653874 [Herbaspirillum huttiense]
MNKPVMPEEESQDKYSVPGLERGLRLLCEFSRQDKTLSAPELARRLDIPRSTVFRLLATLERMGFVERADGGRDYRLGMS
VLRLGFEYLASLELTEIGRPLLDRLRDEIGYSCNLVVRDGRSIVYVAKSVTQTAFTSHVNVGTRLPAHATVLGRVLLEDL
TLPQLRTLYPEEHLEVFSSNTPKTVVELFDMVQADKERGFVLQEGFFETSISTVAAPVRDHSGHVVAALGATIPSPHIDA
SQLDVIVSRVRETAAELSRLLDHAPERGAKVVNMWRG

Nucleotide

• Length: 834 bp