Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 298210..298862 Replicon chromosome
Accession NZ_CP103866
Organism Laceyella sacchari strain FBKL4.014

Toxin (Protein)


Gene name mazF Uniprot ID A0A7W2AID8
Locus tag NYR52_RS01630 Protein ID WP_022738671.1
Coordinates 298512..298862 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2I7SWQ4
Locus tag NYR52_RS01625 Protein ID WP_035952874.1
Coordinates 298210..298506 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYR52_RS01595 (NYR52_01595) 293701..294156 - 456 WP_259436157.1 DUF2269 domain-containing protein -
NYR52_RS01600 (NYR52_01600) 294209..294496 - 288 WP_132223499.1 antibiotic biosynthesis monooxygenase family protein -
NYR52_RS01605 (NYR52_01605) 294684..295067 + 384 WP_132223502.1 holo-ACP synthase -
NYR52_RS01610 (NYR52_01610) 295263..296291 + 1029 WP_259436158.1 outer membrane lipoprotein carrier protein LolA -
NYR52_RS01615 (NYR52_01615) 296410..296838 + 429 WP_132223508.1 hypothetical protein -
NYR52_RS01620 (NYR52_01620) 296843..298051 + 1209 WP_259436159.1 alanine racemase -
NYR52_RS01625 (NYR52_01625) 298210..298506 + 297 WP_035952874.1 ribbon-helix-helix protein, CopG family Antitoxin
NYR52_RS01630 (NYR52_01630) 298512..298862 + 351 WP_022738671.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NYR52_RS01635 (NYR52_01635) 299065..300069 + 1005 WP_132223514.1 PP2C family protein-serine/threonine phosphatase -
NYR52_RS01640 (NYR52_01640) 300092..300427 + 336 WP_022738669.1 STAS domain-containing protein -
NYR52_RS01645 (NYR52_01645) 300424..300909 + 486 WP_022738667.1 anti-sigma B factor RsbW -
NYR52_RS01650 (NYR52_01650) 300875..301660 + 786 WP_022738666.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-50)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12912.98 Da        Isoelectric Point: 5.1224

>T256871 WP_022738671.1 NZ_CP103866:298512-298862 [Laceyella sacchari]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKAYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMSRVNEALMISLGMIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11347.24 Da        Isoelectric Point: 6.3443

>AT256871 WP_035952874.1 NZ_CP103866:298210-298506 [Laceyella sacchari]
MIGWGCFILSGTKRIMISLPTNLLQEVDGMVARDNSNRSEFIRQAMKMYLQERKKRLIREMMQRGYMEMARINLNIASEA
FVAEEEAEDTLDRLVSGV

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2AID8

Antitoxin

Source ID Structure
AlphaFold DB A0A2I7SWQ4

References