Detailed information of TA system TA025687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 2024732..2025498
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MEALZ_RS08590
• Protein ID: WP_046061067.1
• Coordinates: 2024732..2025229 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: G4T110
• Locus tag: MEALZ_RS08595
• Protein ID: WP_014148237.1
• Coordinates: 2025226..2025498 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS08555	2019909..2021150	-	1242	WP_014148230.1	hypothetical protein	-
MEALZ_RS08560	2021234..2021434	-	201	WP_014148231.1	hypothetical protein	-
MEALZ_RS08565	2021724..2022173	+	450	WP_014148232.1	hypothetical protein	-
MEALZ_RS21780	2022469..2022708	+	240	WP_084685383.1	hypothetical protein	-
MEALZ_RS08570	2022934..2023245	+	312	WP_014148233.1	transcriptional regulator	-
MEALZ_RS08575	2023238..2023525	+	288	WP_014148234.1	type II toxin-antitoxin system MqsA family antitoxin	-
MEALZ_RS08580	2023892..2024146	+	255	WP_014148235.1	hypothetical protein	-
MEALZ_RS08585	2024146..2024463	+	318	WP_046061066.1	type II toxin-antitoxin system PemK/MazF family toxin	-
MEALZ_RS08590	2024732..2025229	-	498	WP_046061067.1	GNAT family N-acetyltransferase	Toxin
MEALZ_RS08595	2025226..2025498	-	273	WP_014148237.1	DUF1778 domain-containing protein	Antitoxin
MEALZ_RS08605	2026100..2027335	+	1236	WP_014148238.1	hypothetical protein	-
MEALZ_RS08610	2027360..2028028	+	669	Protein_1679	transporter substrate-binding protein	-
MEALZ_RS08615	2028029..2029256	-	1228	Protein_1680	ISL3 family transposase	-
MEALZ_RS08620	2029298..2029939	+	642	Protein_1681	transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2028082..2028900	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18573.40 Da
• Isoelectric Point: 9.7297

>T25687 WP_046061067.1 NC_016112:c2025229-2024732 [Methylotuvimicrobium alcaliphilum 20Z]
IMRTVLLNKARHDRNRFNCGVEPLNNYLKVMASQQAKKDSTRTFILEDEASESKIVGFYTLTMTPVDLKALPSQLQKKHP
SSISGGLIARLAVDERYKGKGYGEWLLLDALKKLLQASDSVGFPIVIVDAKDGAKHFYEKYGFTTFRDAENKLFITIADI
RSSLD

Nucleotide

• Length: 498 bp

>T25687 NC_016112:c2025229-2024732 [Methylotuvimicrobium alcaliphilum 20Z]
ATAATGAGAACCGTACTTTTAAACAAAGCAAGACACGATAGAAACCGATTCAATTGCGGTGTTGAACCTTTAAACAACTA
CCTAAAAGTCATGGCTAGTCAACAAGCCAAGAAAGACAGTACAAGGACTTTCATACTCGAAGACGAAGCCAGCGAATCCA
AAATTGTTGGCTTTTATACGCTAACGATGACTCCCGTCGATTTAAAAGCTTTACCTTCACAGCTTCAGAAAAAACATCCA
TCATCAATCTCTGGTGGATTGATTGCTCGATTGGCTGTCGACGAGCGTTACAAAGGAAAAGGCTACGGTGAATGGTTGCT
CCTTGACGCACTCAAAAAACTGCTTCAAGCAAGCGATTCCGTCGGGTTTCCGATAGTTATCGTTGACGCCAAAGACGGAG
CCAAGCATTTTTACGAAAAGTATGGATTCACTACTTTTAGAGATGCCGAAAACAAGCTGTTTATCACCATTGCCGACATA
CGGTCCAGCTTGGATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9697.18 Da
• Isoelectric Point: 5.7808

>AT25687 WP_014148237.1 NC_016112:c2025498-2025226 [Methylotuvimicrobium alcaliphilum 20Z]
MATILPRITARVDIDTQDLLTRAAAISGMPSINSFVLSAAVEKAKQIIEQDKALKLTEHDAMLLMDALDKPATANSNLKA
AAARYENTTQ

Nucleotide

• Length: 273 bp

>AT25687 NC_016112:c2025498-2025226 [Methylotuvimicrobium alcaliphilum 20Z]
ATGGCGACTATATTACCTCGTATCACCGCTCGGGTGGATATTGATACTCAGGATTTACTCACTAGAGCTGCGGCTATATC
CGGCATGCCTAGTATTAACTCATTTGTCCTTAGCGCGGCAGTTGAAAAAGCCAAACAAATTATTGAACAAGACAAAGCAC
TTAAACTAACTGAGCATGATGCCATGTTGCTTATGGATGCGCTTGATAAACCTGCAACGGCTAACAGTAACTTAAAAGCA
GCGGCTGCCCGTTATGAGAACACAACTCAATAA

Structures

Antitoxin

• AlphaFold DB: G4T110