Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2480695..2481192 Replicon chromosome
Accession NZ_CP103864
Organism Escherichia coli O157:H7 strain RV06

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NUE47_RS12075 Protein ID WP_001173343.1
Coordinates 2481019..2481192 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NUE47_RS12070 Protein ID WP_000398619.1
Coordinates 2480695..2480991 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE47_RS12050 (2477426) 2477426..2478145 - 720 WP_000611288.1 two-component system response regulator DcuR -
NUE47_RS12055 (2478142) 2478142..2479773 - 1632 WP_001216477.1 sensor histidine kinase -
NUE47_RS12060 (2479954) 2479954..2480184 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NUE47_RS12065 (2480196) 2480196..2480468 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
NUE47_RS12070 (2480695) 2480695..2480991 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NUE47_RS12075 (2481019) 2481019..2481192 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NUE47_RS12080 (2481311) 2481311..2482828 - 1518 WP_001295074.1 lysine--tRNA ligase -
NUE47_RS12085 (2483065) 2483065..2484522 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T256855 WP_001173343.1 NZ_CP103864:2481019-2481192 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT256855 WP_000398619.1 NZ_CP103864:2480695-2480991 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References