Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1629278..1630078 Replicon chromosome
Accession NZ_CP103864
Organism Escherichia coli O157:H7 strain RV06

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag NUE47_RS08095 Protein ID WP_000342448.1
Coordinates 1629278..1629805 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NUE47_RS08100 Protein ID WP_001277108.1
Coordinates 1629812..1630078 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE47_RS08070 (1624353) 1624353..1625120 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
NUE47_RS08075 (1625117) 1625117..1626394 - 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
NUE47_RS08080 (1626391) 1626391..1627317 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NUE47_RS08085 (1627365) 1627365..1628474 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NUE47_RS08090 (1628898) 1628898..1629281 + 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
NUE47_RS08095 (1629278) 1629278..1629805 - 528 WP_000342448.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NUE47_RS08100 (1629812) 1629812..1630078 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NUE47_RS08105 (1630228) 1630228..1631331 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NUE47_RS08110 (1631602) 1631602..1632456 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NUE47_RS08115 (1632701) 1632701..1633759 - 1059 WP_001042018.1 permease-like cell division protein FtsX -
NUE47_RS08120 (1633752) 1633752..1634420 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T256851 WP_000342448.1 NZ_CP103864:c1629805-1629278 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT256851 WP_001277108.1 NZ_CP103864:c1630078-1629812 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References