Detailed information of TA system TA025685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 2023892..2024463
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4T108
• Locus tag: MEALZ_RS08580
• Protein ID: WP_014148235.1
• Coordinates: 2023892..2024146 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MEALZ_RS08585
• Protein ID: WP_046061066.1
• Coordinates: 2024146..2024463 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS08540	2019174..2019380	+	207	WP_014148229.1	hypothetical protein	-
MEALZ_RS08545	2019373..2019594	+	222	WP_046061064.1	type II toxin-antitoxin system HicA family toxin	-
MEALZ_RS08550	2019665..2019907	+	243	WP_046061065.1	hypothetical protein	-
MEALZ_RS08555	2019909..2021150	-	1242	WP_014148230.1	hypothetical protein	-
MEALZ_RS08560	2021234..2021434	-	201	WP_014148231.1	hypothetical protein	-
MEALZ_RS08565	2021724..2022173	+	450	WP_014148232.1	hypothetical protein	-
MEALZ_RS21780	2022469..2022708	+	240	WP_084685383.1	hypothetical protein	-
MEALZ_RS08570	2022934..2023245	+	312	WP_014148233.1	transcriptional regulator	-
MEALZ_RS08575	2023238..2023525	+	288	WP_014148234.1	type II toxin-antitoxin system MqsA family antitoxin	-
MEALZ_RS08580	2023892..2024146	+	255	WP_014148235.1	hypothetical protein	Toxin
MEALZ_RS08585	2024146..2024463	+	318	WP_046061066.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
MEALZ_RS08590	2024732..2025229	-	498	WP_046061067.1	GNAT family N-acetyltransferase	-
MEALZ_RS08595	2025226..2025498	-	273	WP_014148237.1	DUF1778 domain-containing protein	-
MEALZ_RS08605	2026100..2027335	+	1236	WP_014148238.1	hypothetical protein	-
MEALZ_RS08610	2027360..2028028	+	669	Protein_1679	transporter substrate-binding protein	-
MEALZ_RS08615	2028029..2029256	-	1228	Protein_1680	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2028082..2028900	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• mRNA interferase PemK-like (1-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9474.67 Da
• Isoelectric Point: 4.2571

>T25685 WP_014148235.1 NC_016112:2023892-2024146 [Methylotuvimicrobium alcaliphilum 20Z]
MTQLVRIGNSQGIRIPKPLIKQAHLEGKELKLQLVNGGLLITPENGVREGWKEAIEQTISAQGHESLDDEWLDFPLDSDD
ELDW

Nucleotide

• Length: 255 bp

>T25685 NC_016112:2023892-2024146 [Methylotuvimicrobium alcaliphilum 20Z]
ATGACTCAGCTAGTACGAATAGGGAATTCTCAAGGCATAAGGATTCCTAAGCCTTTGATTAAACAAGCTCATTTGGAAGG
AAAAGAGTTAAAATTACAATTGGTTAACGGTGGTCTCCTCATAACCCCGGAAAATGGTGTTAGAGAAGGATGGAAAGAAG
CTATAGAACAAACAATTTCGGCTCAGGGGCATGAGTCTCTAGATGATGAATGGTTGGATTTCCCTCTCGACTCAGATGAT
GAACTGGACTGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11825.01 Da
• Isoelectric Point: 9.0014

>AT25685 WP_046061066.1 NC_016112:2024146-2024463 [Methylotuvimicrobium alcaliphilum 20Z]
MIHRFEVWLADLNPTKGTEINKTRPCVIISPNEMSALSTVLMAPMTTKGFEFPCRVQCKFKGKSGLILLDQIRAVDKSRL
VKNLGAVSAKTQKELCSCLQEMFEY

Nucleotide

• Length: 318 bp

>AT25685 NC_016112:2024146-2024463 [Methylotuvimicrobium alcaliphilum 20Z]
ATGATACACCGATTTGAAGTTTGGCTCGCTGACCTCAATCCCACAAAAGGAACAGAGATCAATAAAACACGCCCTTGCGT
AATAATTTCTCCCAATGAAATGTCAGCTTTGTCTACGGTCCTTATGGCTCCAATGACTACTAAGGGTTTTGAGTTTCCTT
GTCGTGTTCAGTGTAAGTTCAAAGGAAAAAGTGGTCTGATCCTGCTGGATCAAATTAGGGCAGTTGATAAATCAAGGCTT
GTTAAAAATCTTGGGGCAGTGAGTGCAAAGACACAAAAAGAACTGTGTTCGTGCTTACAAGAAATGTTTGAGTATTAA

Structures

Toxin

• AlphaFold DB: G4T108