Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1091170..1091824 Replicon chromosome
Accession NZ_CP103864
Organism Escherichia coli O157:H7 strain RV06

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NUE47_RS05360 Protein ID WP_000244781.1
Coordinates 1091170..1091577 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NUE47_RS05365 Protein ID WP_000354046.1
Coordinates 1091558..1091824 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE47_RS05340 (1087127) 1087127..1088860 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
NUE47_RS05345 (1088866) 1088866..1089576 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NUE47_RS05350 (1089601) 1089601..1090497 - 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
NUE47_RS05355 (1090609) 1090609..1091130 + 522 WP_001055874.1 flavodoxin FldB -
NUE47_RS05360 (1091170) 1091170..1091577 - 408 WP_000244781.1 protein YgfX Toxin
NUE47_RS05365 (1091558) 1091558..1091824 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NUE47_RS05370 (1092067) 1092067..1093047 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
NUE47_RS05375 (1093124) 1093124..1093783 - 660 WP_000250274.1 hemolysin III family protein -
NUE47_RS05380 (1093947) 1093947..1094258 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
NUE47_RS05385 (1094303) 1094303..1095559 + 1257 WP_009671380.1 family 1 glycosylhydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T256847 WP_000244781.1 NZ_CP103864:c1091577-1091170 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT256847 WP_000354046.1 NZ_CP103864:c1091824-1091558 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References