Detailed information of TA system TA025683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1355264..1356030
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: G4SUR9
• Locus tag: MEALZ_RS05875
• Protein ID: WP_014147694.1
• Coordinates: 1355264..1355761 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: G4SUS0
• Locus tag: MEALZ_RS05880
• Protein ID: WP_014147695.1
• Coordinates: 1355758..1356030 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS21610	1350901..1351215	-	315	Protein_1141	HigA family addiction module antidote protein	-
MEALZ_RS05850	1351226..1351498	-	273	WP_014147689.1	hypothetical protein	-
MEALZ_RS05860	1352887..1353570	+	684	WP_017840065.1	curli production assembly protein CsgG	-
MEALZ_RS05865	1353573..1353971	+	399	WP_014147692.1	DUF4810 domain-containing protein	-
MEALZ_RS05870	1353968..1354597	+	630	WP_014147693.1	DUF799 family lipoprotein	-
MEALZ_RS05875	1355264..1355761	-	498	WP_014147694.1	GNAT family N-acetyltransferase	Toxin
MEALZ_RS05880	1355758..1356030	-	273	WP_014147695.1	DUF1778 domain-containing protein	Antitoxin
MEALZ_RS05885	1356212..1358737	-	2526	WP_014147696.1	DNA phosphorothioation-associated putative methyltransferase	-
MEALZ_RS05890	1358753..1359916	-	1164	WP_014147697.1	aminotransferase class V-fold PLP-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (26-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18215.93 Da
• Isoelectric Point: 10.0552

>T25683 WP_014147694.1 NC_016112:c1355761-1355264 [Methylotuvimicrobium alcaliphilum 20Z]
MNLTLSAPQPLAPTHLLNDFECGEHTLDDWLKRRAMSNQLSGASRTFVVVDEGGRVRGYYAMAAGAISHQLATSAIRRNM
PDPVPVMVLGRLAVDRRAQGIKLGAAMLQDAVKRALVVSQNTGVRALLVHALNDRAKQFYEHYGFQESPQHTMTLMLRLN
SYANS

Nucleotide

• Length: 498 bp

>T25683 NC_016112:c1355761-1355264 [Methylotuvimicrobium alcaliphilum 20Z]
ATGAACTTGACGCTATCCGCACCTCAACCGCTTGCTCCCACACACCTATTGAATGATTTCGAGTGCGGTGAACATACACT
GGATGACTGGCTCAAACGCCGAGCGATGAGCAACCAACTCAGCGGAGCCAGTAGAACGTTTGTGGTCGTTGATGAAGGTG
GGCGTGTGCGGGGTTACTACGCCATGGCTGCTGGCGCGATATCCCACCAATTGGCGACGAGTGCAATTCGCCGCAACATG
CCTGATCCCGTTCCGGTCATGGTGCTTGGCAGGTTGGCCGTAGATCGCCGGGCGCAGGGTATTAAGTTGGGCGCCGCCAT
GCTGCAAGACGCCGTAAAACGTGCGCTTGTCGTATCACAAAACACAGGCGTTCGCGCATTGCTTGTCCATGCCCTCAATG
ATCGTGCGAAACAGTTCTACGAGCATTACGGTTTTCAGGAGTCGCCGCAGCATACTATGACTTTGATGCTACGTTTGAAT
AGCTATGCAAATTCTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10156.68 Da
• Isoelectric Point: 5.0773

>AT25683 WP_014147695.1 NC_016112:c1356030-1355758 [Methylotuvimicrobium alcaliphilum 20Z]
MRAASINLRALPEQRDLIDHAANLLGKNRSDFMLEAACERAQSVLLDQVFFGMDADQFQKFTAMLDAPPKPNVGLERLMA
VKPPWENNKE

Nucleotide

• Length: 273 bp

>AT25683 NC_016112:c1356030-1355758 [Methylotuvimicrobium alcaliphilum 20Z]
ATGCGCGCAGCTTCTATCAATCTACGTGCCTTGCCGGAACAGCGAGATCTCATTGATCATGCAGCTAATCTGCTAGGCAA
AAACCGTTCGGACTTCATGCTGGAAGCTGCTTGTGAACGTGCCCAGTCGGTTTTACTTGACCAAGTCTTTTTCGGCATGG
ATGCGGATCAATTCCAAAAGTTCACTGCAATGCTAGACGCGCCGCCGAAGCCCAATGTCGGCCTGGAGCGACTGATGGCG
GTTAAGCCGCCTTGGGAAAATAATAAAGAATGA

Structures

Toxin

• AlphaFold DB: G4SUR9

Antitoxin

• AlphaFold DB: G4SUS0