Detailed information of TA system TA025682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1206666..1207149
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: G4ST47
• Locus tag: MEALZ_RS05195
• Protein ID: WP_014147556.1
• Coordinates: 1206874..1207149 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: MEALZ_RS05190
• Protein ID: WP_046061002.1
• Coordinates: 1206666..1206890 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS05165	1202269..1203480	+	1212	WP_014147551.1	hypothetical protein	-
MEALZ_RS05170	1203494..1204378	+	885	WP_046061001.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
MEALZ_RS21555	1204547..1204744	+	198	WP_084685329.1	hypothetical protein	-
MEALZ_RS05175	1204733..1204981	-	249	WP_014147553.1	hypothetical protein	-
MEALZ_RS05180	1205055..1205708	+	654	WP_014147554.1	hypothetical protein	-
MEALZ_RS05185	1205701..1206624	+	924	WP_014147555.1	ATP-binding protein	-
MEALZ_RS05190	1206666..1206890	+	225	WP_046061002.1	TraY domain-containing protein	Antitoxin
MEALZ_RS05195	1206874..1207149	+	276	WP_014147556.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MEALZ_RS05200	1207264..1207587	-	324	WP_014147557.1	hypothetical protein	-
MEALZ_RS05205	1207635..1208309	+	675	Protein_1014	metal-dependent hydrolase	-
MEALZ_RS21560	1208319..1209551	-	1233	WP_014147559.1	hypothetical protein	-
MEALZ_RS05210	1209644..1209844	-	201	WP_014147560.1	hypothetical protein	-
MEALZ_RS05215	1210120..1210572	+	453	Protein_1017	metal-dependent hydrolase	-
MEALZ_RS05220	1210841..1211266	+	426	WP_014147562.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10507.38 Da
• Isoelectric Point: 10.6468

>T25682 WP_014147556.1 NC_016112:1206874-1207149 [Methylotuvimicrobium alcaliphilum 20Z]
MTWKIEFDPAAERELAKLDKAVARRILKFLTERLAPSDNPRSLGAPLAGSKLGEFWKYRIGKYRVIASIEDRIMTVLVLK
VDKRSDVYKQK

Nucleotide

• Length: 276 bp

>T25682 NC_016112:1206874-1207149 [Methylotuvimicrobium alcaliphilum 20Z]
ATGACCTGGAAGATTGAATTCGATCCGGCAGCCGAACGCGAACTGGCGAAGCTGGACAAGGCTGTTGCCCGCCGGATTTT
AAAGTTTTTGACCGAGCGACTGGCGCCATCGGACAACCCGCGTAGTCTAGGCGCTCCGCTGGCCGGTTCTAAACTCGGCG
AGTTCTGGAAATACCGCATCGGCAAATACCGTGTCATCGCCAGCATCGAGGATCGGATAATGACGGTTCTGGTGTTGAAA
GTGGATAAGCGCAGCGATGTGTACAAACAAAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8738.96 Da
• Isoelectric Point: 4.6558

>AT25682 WP_046061002.1 NC_016112:1206666-1206890 [Methylotuvimicrobium alcaliphilum 20Z]
MLAIRLPDELEKRLDNLAKLTGRTKTFYARQAIEQHLDDLEDLYLAEQRLTEIRAGRTQPIPLEDVLKRYDLED

Nucleotide

• Length: 225 bp

>AT25682 NC_016112:1206666-1206890 [Methylotuvimicrobium alcaliphilum 20Z]
ATGTTAGCAATTCGATTGCCGGACGAGCTGGAAAAACGCTTGGACAATTTAGCCAAACTCACGGGCCGCACGAAAACCTT
TTACGCCAGACAAGCCATCGAGCAGCACCTGGACGACTTGGAAGACCTGTATCTGGCTGAACAGCGCCTGACCGAGATTC
GCGCCGGACGCACGCAACCCATTCCGCTCGAGGACGTGCTGAAGCGCTATGACCTGGAAGATTGA

Structures

Toxin

• AlphaFold DB: G4ST47