256806

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 487425..488062 Replicon chromosome
Accession NZ_CP103856
Organism Bacillus velezensis strain SRCM116265

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NX081_RS02470 Protein ID WP_003156187.1
Coordinates 487712..488062 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NX081_RS02465 Protein ID WP_003156188.1
Coordinates 487425..487706 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX081_RS02445 (483790) 483790..484389 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NX081_RS02450 (484482) 484482..484847 + 366 WP_007410229.1 holo-ACP synthase -
NX081_RS02455 (485012) 485012..486019 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
NX081_RS02460 (486136) 486136..487305 + 1170 WP_025284272.1 alanine racemase -
NX081_RS02465 (487425) 487425..487706 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NX081_RS02470 (487712) 487712..488062 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NX081_RS02475 (488180) 488180..489001 + 822 WP_003156182.1 STAS domain-containing protein -
NX081_RS02480 (489006) 489006..489371 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NX081_RS02485 (489374) 489374..489775 + 402 WP_003156178.1 anti-sigma regulatory factor -
NX081_RS02490 (489787) 489787..490794 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
NX081_RS02495 (490858) 490858..491187 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NX081_RS02500 (491184) 491184..491666 + 483 WP_007609591.1 anti-sigma B factor RsbW -
NX081_RS02505 (491632) 491632..492420 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NX081_RS02510 (492420) 492420..493022 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T256806 WP_003156187.1 NZ_CP103856:487712-488062 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT256806 WP_003156188.1 NZ_CP103856:487425-487706 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References