Detailed information of TA system TA025680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 339523..340031
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G4SWU7
• Locus tag: MEALZ_RS01535
• Protein ID: WP_014146822.1
• Coordinates: 339771..340031 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G4SWU6
• Locus tag: MEALZ_RS01530
• Protein ID: WP_014146821.1
• Coordinates: 339523..339774 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS01525	337092..339170	+	2079	WP_014146820.1	heavy metal translocating P-type ATPase	-
MEALZ_RS01530	339523..339774	+	252	WP_014146821.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
MEALZ_RS01535	339771..340031	+	261	WP_014146822.1	Txe/YoeB family addiction module toxin	Toxin
MEALZ_RS01540	340335..340796	-	462	WP_014146823.1	CreA family protein	-
MEALZ_RS01545	341163..342731	-	1569	WP_084685238.1	sigma 54-interacting transcriptional regulator	-
MEALZ_RS21325	342804..343046	-	243	WP_084685240.1	zinc ribbon domain-containing protein	-
MEALZ_RS01550	343138..343791	-	654	WP_014146825.1	carbonic anhydrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10454.08 Da
• Isoelectric Point: 9.7863

>T25680 WP_014146822.1 NC_016112:339771-340031 [Methylotuvimicrobium alcaliphilum 20Z]
MKLTFSSNAWENYLYWQKTDKAMLKRVNMLIKDIQRSPFEGIGKPEPLKHGLSGYWSRRINDEHRLVYKVVEVDIFIAQL
RYHYEY

Nucleotide

• Length: 261 bp

>T25680 NC_016112:339771-340031 [Methylotuvimicrobium alcaliphilum 20Z]
ATGAAACTCACTTTCTCAAGCAACGCGTGGGAAAACTACTTATATTGGCAAAAAACCGATAAAGCCATGCTTAAACGAGT
CAATATGCTGATTAAAGATATTCAGCGTTCGCCATTTGAAGGAATAGGGAAGCCGGAGCCTTTGAAGCATGGCCTGTCCG
GTTACTGGTCCAGGCGAATCAACGATGAGCATAGGCTGGTATACAAAGTAGTCGAAGTTGACATTTTTATTGCCCAGCTT
CGCTACCACTACGAATATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9333.65 Da
• Isoelectric Point: 4.6749

>AT25680 WP_014146821.1 NC_016112:339523-339774 [Methylotuvimicrobium alcaliphilum 20Z]
MDAISYTAARANLAKTMEKVCNDHEPVIITRKRESPVVMISLEDFQSMEETAYLLRSPANARHLLESIAELEAGKGTERE
LLE

Nucleotide

• Length: 252 bp

>AT25680 NC_016112:339523-339774 [Methylotuvimicrobium alcaliphilum 20Z]
ATGGATGCCATCAGTTATACAGCAGCCCGCGCTAATCTTGCGAAAACAATGGAAAAGGTTTGCAATGATCATGAGCCGGT
GATTATTACTCGAAAAAGAGAATCTCCCGTCGTTATGATTTCCTTGGAAGACTTTCAGTCCATGGAAGAGACGGCATATT
TGCTACGCTCGCCTGCAAACGCTCGTCACCTCCTTGAATCGATAGCGGAACTCGAAGCAGGAAAAGGTACCGAGCGAGAA
TTGTTGGAATGA

Structures

Toxin

• AlphaFold DB: G4SWU7

Antitoxin

• AlphaFold DB: G4SWU6