Detailed information of TA system TA025679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/Txe-YefM
• Location: 314158..314669
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: G4SWS5
• Locus tag: MEALZ_RS01430
• Protein ID: WP_014146800.1
• Coordinates: 314158..314430 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G4SWS6
• Locus tag: MEALZ_RS01435
• Protein ID: WP_014146801.1
• Coordinates: 314427..314669 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS22515	309765..309932	+	168	WP_162472901.1	hypothetical protein	-
MEALZ_RS01405	310425..311270	-	846	WP_014146796.1	2-oxoacid:ferredoxin oxidoreductase subunit beta	-
MEALZ_RS01410	311267..313012	-	1746	WP_014146797.1	2-oxoacid:acceptor oxidoreductase subunit alpha	-
MEALZ_RS01420	313363..313680	-	318	WP_046060928.1	type II toxin-antitoxin system PemK/MazF family toxin	-
MEALZ_RS01425	313680..313934	-	255	WP_014146799.1	hypothetical protein	-
MEALZ_RS01430	314158..314430	-	273	WP_014146800.1	Txe/YoeB family addiction module toxin	Toxin
MEALZ_RS01435	314427..314669	-	243	WP_014146801.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MEALZ_RS22520	315012..315182	-	171	WP_014146802.1	hypothetical protein	-
MEALZ_RS01440	315303..316349	-	1047	WP_046060929.1	DUF4917 family protein	-
MEALZ_RS20535	316479..316922	-	444	WP_014146804.1	DUF4326 domain-containing protein	-
MEALZ_RS01450	317030..317245	-	216	WP_014146805.1	DUF2283 domain-containing protein	-
MEALZ_RS01455	317368..317784	-	417	WP_014146806.1	hypothetical protein	-
MEALZ_RS01460	317759..318145	-	387	WP_014146807.1	helix-turn-helix transcriptional regulator	-
MEALZ_RS01465	318146..318559	-	414	WP_014146808.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MEALZ_RS01470	318679..319362	-	684	WP_014146809.1	ribonucleotide reductase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10551.41 Da
• Isoelectric Point: 10.7031

>T25679 WP_014146800.1 NC_016112:c314430-314158 [Methylotuvimicrobium alcaliphilum 20Z]
VTFTAWKLVYTKQAQRNAKKLASSGLKLKAQKLVELIAKDPYRKPPPFEKLIGDLTGAYSRRINIQHRLVYQVLEDERVV
KVLRLWSHYE

Nucleotide

• Length: 273 bp

>T25679 NC_016112:c314430-314158 [Methylotuvimicrobium alcaliphilum 20Z]
GTGACATTTACCGCATGGAAGTTGGTTTACACCAAGCAAGCTCAGAGGAATGCAAAGAAGTTGGCCTCCAGCGGCCTGAA
GCTAAAAGCACAGAAATTGGTGGAGCTGATAGCAAAAGATCCGTATCGTAAGCCGCCTCCGTTTGAGAAGCTCATCGGCG
ATCTTACGGGAGCCTATTCCCGCCGGATCAATATTCAGCATCGCCTAGTGTACCAAGTACTTGAAGATGAACGAGTAGTT
AAAGTTCTTCGGCTCTGGAGCCACTACGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9053.17 Da
• Isoelectric Point: 4.2340

>AT25679 WP_014146801.1 NC_016112:c314669-314427 [Methylotuvimicrobium alcaliphilum 20Z]
MTGITATEARSNLYRLIDETAESHQPIVITGKRNKAVLVSEEDWSAIQETLYLLSVPGMRESIRKGMDTPVDECNEELDW

Nucleotide

• Length: 243 bp

>AT25679 NC_016112:c314669-314427 [Methylotuvimicrobium alcaliphilum 20Z]
ATGACCGGAATAACTGCAACTGAGGCGCGCAGCAACCTTTATCGGTTGATTGATGAAACCGCCGAATCTCACCAACCAAT
CGTCATCACGGGTAAACGGAATAAAGCTGTTCTGGTTTCCGAAGAAGATTGGTCTGCAATTCAGGAAACGCTTTACCTGC
TATCCGTACCAGGCATGCGTGAGTCTATTCGGAAGGGTATGGATACTCCTGTGGATGAATGCAATGAAGAGCTCGACTGG
TGA

Structures

Toxin

• AlphaFold DB: G4SWS5

Antitoxin

• AlphaFold DB: G4SWS6