Detailed information of TA system TA025673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 91710..92263
• Replicon: plasmid MEALZ_p
• Accession: NC_016108
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G4T4N9
• Locus tag: MEALZ_RS20360
• Protein ID: WP_014133151.1
• Coordinates: 91970..92263 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MEALZ_RS20355
• Protein ID: WP_046061738.1
• Coordinates: 91710..91982 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS22900	87611..87778	-	168	WP_014133144.1	hypothetical protein	-
MEALZ_RS20330	87981..88616	+	636	WP_014133145.1	hypothetical protein	-
MEALZ_RS20335	88736..89350	+	615	WP_014133146.1	hypothetical protein	-
MEALZ_RS20340	89354..90073	-	720	WP_014133147.1	metal-dependent hydrolase	-
MEALZ_RS20345	90229..90444	+	216	WP_084685675.1	hypothetical protein	-
MEALZ_RS20350	90548..91516	-	969	WP_014133149.1	tyrosine-type recombinase/integrase	-
MEALZ_RS20355	91710..91982	+	273	WP_046061738.1	CopG family ribbon-helix-helix protein	Antitoxin
MEALZ_RS20360	91970..92263	+	294	WP_014133151.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MEALZ_RS20365	92493..94838	-	2346	WP_014133152.1	S8 family peptidase	-
MEALZ_RS20370	94828..95991	-	1164	WP_014133153.1	AAA family ATPase	-
MEALZ_RS20375	96188..96730	+	543	WP_048481421.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..128415	128415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11048.62 Da
• Isoelectric Point: 4.8603

>T25673 WP_014133151.1 NC_016108:91970-92263 [Methylotuvimicrobium alcaliphilum 20Z]
VPRIIITEGATQGLERCRLFLAEKSPLAAARAARAIAQQFELLETEPEIGRPWEEFPQLRELLIPFGDSGFVALYRYEPN
DNALYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T25673 NC_016108:91970-92263 [Methylotuvimicrobium alcaliphilum 20Z]
GTGCCACGAATAATAATTACCGAAGGAGCGACTCAAGGTTTGGAGCGCTGTCGCCTATTTTTAGCCGAAAAAAGTCCTTT
GGCTGCGGCAAGAGCCGCGCGGGCAATTGCACAGCAATTTGAGCTCCTAGAAACAGAACCCGAAATCGGCAGGCCGTGGG
AAGAATTTCCACAGTTGCGTGAATTACTCATTCCATTCGGCGATTCCGGTTTTGTGGCACTCTATCGCTATGAACCGAAT
GATAATGCTCTTTATGTACTTGCCTTTCGACACCAGAAAGAAGCGGGTTACTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10699.79 Da
• Isoelectric Point: 6.1320

>AT25673 WP_046061738.1 NC_016108:91710-91982 [Methylotuvimicrobium alcaliphilum 20Z]
MATSVKLDADLKSRIQHLADLRHRSSHWIMREAIRDYVEREEARESFKQEALSAWQAYQETGKHLTGQEVRHWLSTWGTE
HEQESPECHE

Nucleotide

• Length: 273 bp

>AT25673 NC_016108:91710-91982 [Methylotuvimicrobium alcaliphilum 20Z]
ATGGCAACATCTGTTAAACTCGATGCGGATTTGAAAAGTCGAATTCAGCATCTGGCTGATCTGCGGCACCGGTCATCGCA
TTGGATTATGCGCGAAGCCATTCGCGATTACGTTGAACGCGAAGAAGCTCGAGAAAGTTTCAAACAAGAAGCCTTGTCAG
CTTGGCAGGCTTATCAAGAAACGGGTAAACACCTAACGGGCCAAGAAGTGCGTCATTGGCTCAGCACCTGGGGTACAGAG
CATGAGCAAGAGAGCCCAGAGTGCCACGAATAA

Structures

Toxin

• AlphaFold DB: G4T4N9