Detailed information of TA system TA256717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1929102..1930759
• Replicon: chromosome
• Accession: NZ_CP103815
• Organism: Actinobacillus equuli subsp. haemolyticus strain 4038

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: NYR69_RS08990
• Protein ID: WP_279438360.1
• Coordinates: 1929428..1930759 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A3N2X7
• Locus tag: NYR69_RS08985
• Protein ID: WP_005613259.1
• Coordinates: 1929102..1929431 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NYR69_RS08970 (NYR69_08975)	1924165..1925445	+	1281	WP_278226526.1	threonine synthase	-
NYR69_RS08975 (NYR69_08980)	1925875..1927140	+	1266	WP_279438358.1	pentapeptide repeat-containing protein	-
NYR69_RS08980 (NYR69_08985)	1927438..1928763	-	1326	WP_279438359.1	MFS transporter family glucose-6-phosphate receptor UhpC	-
NYR69_RS08985 (NYR69_08990)	1929102..1929431	-	330	WP_005613259.1	helix-turn-helix domain-containing protein	Antitoxin
NYR69_RS08990 (NYR69_08995)	1929428..1930759	-	1332	WP_279438360.1	HipA domain-containing protein	Toxin
NYR69_RS08995 (NYR69_09000)	1931161..1932189	+	1029	WP_279438361.1	Abi family protein	-
NYR69_RS09000 (NYR69_09005)	1932418..1932807	-	390	WP_279438362.1	hypothetical protein	-
NYR69_RS09005 (NYR69_09010)	1932810..1933319	-	510	WP_279438363.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (164-387)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51183.05 Da
• Isoelectric Point: 5.2284

>T256717 WP_279438360.1 NZ_CP103815:c1930759-1929428 [Actinobacillus equuli subsp. haemolyticus]
MSDISVKNKKLTLQTYVDNLWRDMAELYFNAQYELESMEYLTDYAVDFFGQANARSISINYPVNVFIDHVDKLMTTLADI
IPAGASRRYWLEALDIKHLSISHQNYLLLKNGAISPIGNVRVKEAIPEIQSERYFPLNVVQNRQSDFLEYANQTGAIVGG
ATGAGGEAPKLLLKRQDKQVWIDNLQLGLDCDTAYLVKYPRGNYSELDCDILRAEYHYYHELRALGFSTIDTDLMKLEEG
QRYPSLWLPRFDMKFKDGKWERYAMESVYSMLQKYGGNLDHEQTIRNLLNVISASQFSNDFDNRHFVIEWVKRDLLNIAF
GNSDNHGRNTAFLRDDNRIWLSPIYDFAPMRADPEGIIRTTTWAKNGDNSQEFAGEYRFDKIADSLSDLIEPDILLRELR
MLATQLVDLKSRLTKRGVPKSILDFPAIGFDYLPEKLQRWGLV

Nucleotide

• Length: 1332 bp

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12320.45 Da
• Isoelectric Point: 10.8292

>AT256717 WP_005613259.1 NZ_CP103815:c1929431-1929102 [Actinobacillus equuli subsp. haemolyticus]
MTIQHKNASPTRSKVNGIERQLIRETIIVQLLKGEISQGQALRRLRVEALGINQQDYLKLAKVSRQTLSNIENDKGNYSI
ETINQVFKPMGLKLGLMPISKDLMASFLK

Nucleotide

• Length: 330 bp

Structures

Antitoxin

• AlphaFold DB: A3N2X7