Detailed information of TA system TA025671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1831811..1832373
• Replicon: chromosome
• Accession: NC_016078
• Organism: Pelagibacterium halotolerans B2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G4REA2
• Locus tag: KKY_RS08995
• Protein ID: WP_014131015.1
• Coordinates: 1831811..1832182 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G4REA3
• Locus tag: KKY_RS09000
• Protein ID: WP_014131016.1
• Coordinates: 1832179..1832373 (-)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKY_RS08960	1827885..1828646	-	762	WP_014131008.1	flagellar type III secretion system protein FliR	-
KKY_RS08965	1828653..1828919	-	267	WP_014131009.1	flagellar biosynthesis protein FliQ	-
KKY_RS08970	1829101..1829424	-	324	WP_014131010.1	flagellar hook-basal body complex protein FliE	-
KKY_RS08975	1829437..1829850	-	414	WP_014131011.1	flagellar basal body rod protein FlgC	-
KKY_RS08980	1829862..1830266	-	405	WP_014131012.1	flagellar basal body rod protein FlgB	-
KKY_RS08990	1830912..1831814	+	903	WP_083824018.1	flagellar type III secretion system pore protein FliP	-
KKY_RS08995	1831811..1832182	-	372	WP_014131015.1	PIN domain-containing protein	Toxin
KKY_RS09000	1832179..1832373	-	195	WP_014131016.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KKY_RS09005	1832427..1835714	-	3288	WP_139305022.1	hypothetical protein	-
KKY_RS09010	1835866..1836615	-	750	WP_014131018.1	hypothetical protein	-
KKY_RS09015	1836612..1837022	-	411	WP_014131019.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-110)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13592.79 Da
• Isoelectric Point: 6.0766

>T25671 WP_014131015.1 NC_016078:c1832182-1831811 [Pelagibacterium halotolerans B2]
MILVDTSVWIDHFRAVDEELELLLGANRVLMHPFVLGELALGNLRQRNLILTSLADLPQCVVARDDEVLEFISLHALFGR
GIGYVDAHLLSAAALTPEARLATRDRRLRDRAQSLGLAAALAR

Nucleotide

• Length: 372 bp

>T25671 NC_016078:c1832182-1831811 [Pelagibacterium halotolerans B2]
ATGATCCTTGTGGACACTTCCGTATGGATCGACCATTTTCGGGCGGTCGATGAGGAATTGGAGCTGCTGTTGGGCGCCAA
CAGGGTGCTGATGCATCCGTTTGTTCTCGGCGAACTTGCGCTGGGGAACCTGCGGCAACGCAATCTCATACTCACTTCGC
TGGCCGACCTGCCCCAGTGCGTCGTCGCCAGAGACGATGAAGTTCTCGAATTTATCAGTCTTCACGCATTGTTCGGGCGG
GGGATAGGGTATGTCGACGCGCATCTTCTCTCTGCTGCCGCCCTTACACCGGAGGCCAGGCTTGCCACCCGCGACCGCAG
GCTGAGGGACCGTGCGCAGAGCCTCGGTCTCGCCGCAGCCTTGGCCCGTTAG

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7099.27 Da
• Isoelectric Point: 10.5887

>AT25671 WP_014131016.1 NC_016078:c1832373-1832179 [Pelagibacterium halotolerans B2]
MRTTIAIDDELVEKAKSLTGLNEKSALVREGLKALIERESARRLALLGGTEPQLKDVPRRRTAI

Nucleotide

• Length: 195 bp

>AT25671 NC_016078:c1832373-1832179 [Pelagibacterium halotolerans B2]
ATGCGCACAACAATCGCCATCGACGATGAACTGGTCGAAAAAGCCAAATCTCTTACCGGTCTTAACGAGAAGTCAGCTCT
GGTCAGAGAGGGGCTGAAGGCGCTTATCGAGCGCGAGAGCGCCCGTCGTCTCGCGCTTCTCGGCGGCACTGAGCCTCAGC
TCAAGGACGTACCGCGGCGACGAACAGCTATATGA

Structures

Toxin

• AlphaFold DB: G4REA2

Antitoxin

• AlphaFold DB: G4REA3