Detailed information of TA system TA256706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1942063..1943720
• Replicon: chromosome
• Accession: NZ_CP103812
• Organism: Actinobacillus equuli subsp. haemolyticus strain 3826

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: NYR84_RS08925
• Protein ID: WP_279469813.1
• Coordinates: 1942389..1943720 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: NYR84_RS08920
• Protein ID: WP_279469812.1
• Coordinates: 1942063..1942392 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NYR84_RS08910 (NYR84_08910)	1938994..1940274	+	1281	WP_279469811.1	threonine synthase	-
NYR84_RS08915 (NYR84_08915)	1940399..1941724	-	1326	WP_279455842.1	MFS transporter family glucose-6-phosphate receptor UhpC	-
NYR84_RS08920 (NYR84_08920)	1942063..1942392	-	330	WP_279469812.1	helix-turn-helix domain-containing protein	Antitoxin
NYR84_RS08925 (NYR84_08925)	1942389..1943720	-	1332	WP_279469813.1	HipA domain-containing protein	Toxin
NYR84_RS08930 (NYR84_08930)	1944049..1944870	+	822	WP_279469814.1	Abi family protein	-
NYR84_RS08935 (NYR84_08935)	1944809..1945078	+	270	WP_279469815.1	hypothetical protein	-
NYR84_RS08940 (NYR84_08940)	1945429..1945980	-	552	WP_279469816.1	transposase	-
NYR84_RS08945 (NYR84_08945)	1946230..1947789	-	1560	WP_279469817.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (164-371)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51106.91 Da
• Isoelectric Point: 4.9570

>T256706 WP_279469813.1 NZ_CP103812:c1943720-1942389 [Actinobacillus equuli subsp. haemolyticus]
MSDISIKSNKLTLQTYVDNLWRDMAELYFNAQYELESMEYLTDYAVDFFGQANARSVSINYPVNVFIDHADKLMTTLADI
IPAGASRRYWLEALDIKHLSISHQNYLLLKNGAISPIGNVRVKEAVPEIQSERYFPLNVVQNRQSDFLEYANQTGAIVGG
ATGAGGEAPKLLLKRQDKQVWIDNLQLGLDCDTAYLVKYPRGNYSELDCDILRAEYHYYHELRALGFSTIDTDLMKLEEG
ERYPSLWLPRFDMKFKDGKWERYAMESVYSMLQKYGGNLDHEQTIRNLLNVISASQFSNDFDNRYFVIEWVKRDLLNIAF
GNSDNHGRNTAFLRDDNRIWLSPIYDFAPMRADPEGIIRTTTWAKNGDNPQEFAGEYRFDKIADGLSDLIEPDILLRELR
MLATQLVDLKSRLTKRGVPKSILDFPAIGFDYLPEKLQRWGLV

Nucleotide

• Length: 1332 bp

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12378.55 Da
• Isoelectric Point: 10.8292

>AT256706 WP_279469812.1 NZ_CP103812:c1942392-1942063 [Actinobacillus equuli subsp. haemolyticus]
MTIQHKNSSPTRPKMNGIERQLIRETIIVQLLKGEISQGQALRRLRVEALGINQQDYLKLAKVSRQTLSNIENDKGNYSI
ETLNQVFKPMGLKLGLMPISKDLMASFLK

Nucleotide

• Length: 330 bp