Detailed information of TA system TA025670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 663883..664480
• Replicon: chromosome
• Accession: NC_016078
• Organism: Pelagibacterium halotolerans B2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G4RD98
• Locus tag: KKY_RS03270
• Protein ID: WP_014129873.1
• Coordinates: 663883..664179 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KKY_RS03275
• Protein ID: WP_014129874.1
• Coordinates: 664181..664480 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKY_RS03250	659995..660861	-	867	WP_014129868.1	alpha/beta hydrolase	-
KKY_RS03255	660930..661202	+	273	WP_041528548.1	metal/formaldehyde-sensitive transcriptional repressor	-
KKY_RS03260	661229..662218	+	990	WP_014129870.1	CDF family Co(II)/Ni(II) efflux transporter DmeF	-
KKY_RS03265	662226..663740	-	1515	WP_014129871.1	threonine ammonia-lyase, biosynthetic	-
KKY_RS03270	663883..664179	+	297	WP_014129873.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KKY_RS03275	664181..664480	+	300	WP_014129874.1	putative addiction module antidote protein	Antitoxin
KKY_RS03280	664552..664965	+	414	WP_014129875.1	TlpA family protein disulfide reductase	-
KKY_RS03285	664986..665192	-	207	WP_014129876.1	hypothetical protein	-
KKY_RS03290	665294..665809	-	516	WP_014129877.1	copper chaperone PCu(A)C	-
KKY_RS03295	665838..667127	-	1290	WP_139304996.1	FtsX-like permease family protein	-
KKY_RS03300	667163..667843	-	681	WP_041528550.1	ABC transporter ATP-binding protein	-
KKY_RS03305	667964..668401	-	438	WP_014129880.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10953.67 Da
• Isoelectric Point: 10.5743

>T25670 WP_014129873.1 NC_016078:663883-664179 [Pelagibacterium halotolerans B2]
MIALIKSAVFDNWLRDLRDRRAMARILARLDRFANGNPGDVKAVGGGISEMRIDYGPGYRVYYMQRGPVVIILLSGGDKS
SQKADIARAREIAAQWKD

Nucleotide

• Length: 297 bp

>T25670 NC_016078:663883-664179 [Pelagibacterium halotolerans B2]
ATGATCGCACTGATAAAGAGTGCCGTTTTTGACAATTGGCTGCGTGATCTGCGCGACAGGCGGGCCATGGCGCGCATTCT
GGCGCGGCTGGATCGGTTTGCGAATGGTAATCCCGGTGACGTCAAAGCCGTCGGCGGCGGGATTTCCGAAATGCGGATCG
ATTATGGACCGGGTTACAGGGTTTACTACATGCAGCGGGGCCCGGTCGTCATCATTCTGCTTTCGGGAGGCGATAAGTCC
TCGCAGAAGGCCGATATCGCCCGCGCCAGGGAAATCGCCGCACAGTGGAAGGACTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10696.04 Da
• Isoelectric Point: 4.7914

>AT25670 WP_014129874.1 NC_016078:664181-664480 [Pelagibacterium halotolerans B2]
MTSKETFSRYDSADYLKSEQDIVAYLEAVIEEGGDDPVYIAQALGVVARAHNMSQLARDTGMSREGLYKALSEDGNPSFA
TIVKVAHALGLRLGFKPAA

Nucleotide

• Length: 300 bp

>AT25670 NC_016078:664181-664480 [Pelagibacterium halotolerans B2]
ATGACGAGCAAGGAAACGTTCAGCCGCTATGATAGCGCCGACTATCTCAAGAGCGAGCAAGACATCGTCGCTTATCTCGA
AGCGGTGATCGAAGAAGGCGGCGACGATCCCGTCTATATCGCGCAAGCGCTTGGGGTGGTCGCCCGCGCCCATAACATGA
GCCAGTTGGCACGCGATACCGGAATGAGCCGCGAAGGGCTTTACAAGGCGCTTTCCGAGGACGGAAATCCAAGCTTTGCG
ACGATTGTAAAGGTCGCCCATGCATTGGGGCTGCGTTTGGGATTCAAGCCCGCCGCATGA

Structures

Toxin

• AlphaFold DB: G4RD98