Detailed information of TA system TA025669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 252324..252816
• Replicon: chromosome
• Accession: NC_016078
• Organism: Pelagibacterium halotolerans B2

Toxin (Protein)

• Gene name: -
• Uniprot ID: G4R8M3
• Locus tag: KKY_RS01265
• Protein ID: WP_014129459.1
• Coordinates: 252553..252816 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: G4R813
• Locus tag: KKY_RS01260
• Protein ID: WP_014129458.1
• Coordinates: 252324..252566 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKY_RS01235	247975..248892	+	918	WP_014129453.1	pca operon transcription factor PcaQ	-
KKY_RS01240	248895..249311	-	417	WP_014129454.1	VOC family protein	-
KKY_RS01245	249336..249935	-	600	WP_014129455.1	DUF2062 domain-containing protein	-
KKY_RS01250	250149..251651	+	1503	WP_014129456.1	inorganic phosphate transporter	-
KKY_RS01255	251779..252270	-	492	WP_014129457.1	NUDIX hydrolase	-
KKY_RS01260	252324..252566	-	243	WP_014129458.1	CopG family transcriptional regulator	Antitoxin
KKY_RS01265	252553..252816	-	264	WP_014129459.1	BrnT family toxin	Toxin
KKY_RS01270	252956..254467	+	1512	WP_014129460.1	ATP-binding protein	-
KKY_RS01275	254654..255568	+	915	WP_014129461.1	ROK family protein	-
KKY_RS01280	255674..256399	+	726	WP_014129462.1	copper homeostasis protein CutC	-
KKY_RS01285	256688..257530	+	843	WP_014129463.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9862.16 Da
• Isoelectric Point: 4.8047

>T25669 WP_014129459.1 NC_016078:c252816-252553 [Pelagibacterium halotolerans B2]
MIFEYDPAQSAANLEKHGIDFEAAQALWADELALEIPARTTDEPRFLVIGKIGDRHWAAVITYRGAAVRIISVRRARDQE
IAYYESL

Nucleotide

• Length: 264 bp

>T25669 NC_016078:c252816-252553 [Pelagibacterium halotolerans B2]
ATGATTTTCGAATACGATCCGGCCCAGAGTGCTGCCAACCTGGAAAAGCACGGAATCGATTTCGAAGCCGCGCAAGCACT
GTGGGCGGACGAGTTGGCGCTGGAAATTCCGGCCCGCACCACCGATGAACCTCGATTTCTGGTCATCGGGAAAATCGGCG
ACCGGCACTGGGCGGCAGTCATCACATATCGGGGCGCGGCCGTTCGCATCATATCGGTGCGCCGTGCCCGAGATCAGGAG
ATCGCATATTATGAAAGCCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9150.45 Da
• Isoelectric Point: 8.7682

>AT25669 WP_014129458.1 NC_016078:c252566-252324 [Pelagibacterium halotolerans B2]
MKASEFDTKFEAGEDIEGALDFARARRPNLESRRVNVDFPAWVVEGLDRQAKRLGVTRQSLIKMWIAQKLEHPVDGNPKL

Nucleotide

• Length: 243 bp

>AT25669 NC_016078:c252566-252324 [Pelagibacterium halotolerans B2]
ATGAAAGCCTCTGAATTCGACACCAAGTTCGAAGCCGGCGAGGATATAGAGGGCGCCCTCGATTTTGCGCGGGCGCGCCG
TCCCAATCTCGAATCGCGCCGGGTCAATGTCGATTTTCCCGCGTGGGTCGTGGAGGGGCTGGACCGGCAGGCCAAACGGC
TCGGCGTTACCCGCCAATCGCTTATCAAGATGTGGATTGCGCAAAAGCTCGAACATCCGGTCGACGGCAATCCGAAGCTT
TGA

Structures

Toxin

• AlphaFold DB: G4R8M3

Antitoxin

• AlphaFold DB: G4R813