Detailed information of TA system TA025668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1924816..1925478
• Replicon: chromosome
• Accession: NC_016052
• Organism: Tetragenococcus halophilus NBRC 12172

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A851I0Q0
• Locus tag: TEH_RS09360
• Protein ID: WP_041591998.1
• Coordinates: 1925293..1925478 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A3G5FKS5
• Locus tag: TEH_RS09355
• Protein ID: WP_041591845.1
• Coordinates: 1924816..1925259 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TEH_RS09335 (TEH_19170)	1920362..1921240	-	879	WP_014125286.1	NAD(P)-dependent oxidoreductase	-
TEH_RS09340 (TEH_19190)	1921536..1922789	-	1254	WP_014125288.1	ABC transporter permease	-
TEH_RS09345 (TEH_19200)	1922782..1923681	-	900	WP_014125289.1	ABC transporter ATP-binding protein	-
TEH_RS09350 (TEH_19210)	1923865..1924587	-	723	WP_014125290.1	alpha/beta hydrolase	-
TEH_RS09355 (TEH_19220)	1924816..1925259	-	444	WP_041591845.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
TEH_RS09360 (TEH_19230)	1925293..1925478	-	186	WP_041591998.1	type II toxin-antitoxin system HicA family toxin	Toxin
TEH_RS09365 (TEH_19240)	1925583..1926107	-	525	WP_014125293.1	acyl-CoA thioesterase	-
TEH_RS09370 (TEH_19250)	1926429..1927214	-	786	WP_014125294.1	acetyl-CoA carboxylase carboxyl transferase subunit alpha	-
TEH_RS09375 (TEH_19260)	1927216..1928079	-	864	WP_014125295.1	acetyl-CoA carboxylase, carboxyltransferase subunit beta	-
TEH_RS09380 (TEH_19270)	1928092..1929456	-	1365	WP_014125296.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
TEH_RS09385 (TEH_19280)	1929464..1929883	-	420	WP_180991148.1	3-hydroxyacyl-ACP dehydratase FabZ	-
TEH_RS09390 (TEH_19290)	1929895..1930398	-	504	WP_014125298.1	acetyl-CoA carboxylase biotin carboxyl carrier protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (13-80)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 7004.41 Da
• Isoelectric Point: 10.7944

>T25668 WP_041591998.1 NC_016052:c1925478-1925293 [Tetragenococcus halophilus NBRC 12172]
MPMTQKQMVKYLAKNGWTIVPKQGKGLHVKMCKSNSRPIIIFHGELNKYTERGILKQAGLY

Nucleotide

• Length: 186 bp

>T25668 NC_016052:c1925478-1925293 [Tetragenococcus halophilus NBRC 12172]
ATGCCGATGACACAAAAGCAAATGGTCAAATATCTGGCCAAAAATGGTTGGACAATTGTTCCTAAACAAGGAAAAGGATT
ACATGTTAAAATGTGCAAATCAAATAGTCGTCCAATCATTATTTTTCATGGTGAATTGAACAAATATACCGAAAGAGGAA
TTTTAAAGCAAGCTGGGCTTTATTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16704.72 Da
• Isoelectric Point: 4.2592

>AT25668 WP_041591845.1 NC_016052:c1925259-1924816 [Tetragenococcus halophilus NBRC 12172]
MLVAYPALFYYDASAQAKYFVTFPDFENSATQGKNIQDALEMASEYLGIIVADLIDTQNHVPSSSDINILDLKENDPFKN
DDDFQLEYDPEKSFITMIYVNINNYLGSQNPVKKTLTIPKWADDLGKKHSLNFSKTLEDAIVEKVNH

Nucleotide

• Length: 444 bp

>AT25668 NC_016052:c1925259-1924816 [Tetragenococcus halophilus NBRC 12172]
ATGTTAGTCGCTTATCCTGCTTTATTTTATTATGACGCTTCAGCTCAAGCAAAATATTTTGTTACTTTTCCAGATTTTGA
AAATAGCGCTACTCAAGGGAAAAATATTCAAGACGCACTTGAGATGGCTTCAGAATACCTGGGGATCATTGTTGCTGATT
TGATTGATACACAGAATCATGTTCCAAGTTCCTCAGATATCAATATACTTGATTTAAAAGAAAACGATCCTTTTAAAAAT
GATGATGATTTCCAATTGGAATATGATCCAGAAAAGTCTTTTATTACGATGATATATGTAAACATAAATAACTATTTAGG
TTCACAAAATCCTGTTAAAAAAACTTTAACGATCCCTAAATGGGCAGATGATTTAGGTAAGAAGCACTCTCTTAATTTCT
CAAAAACATTAGAGGATGCTATTGTAGAAAAAGTGAATCATTAG

Structures

Toxin

• AlphaFold DB: A0A851I0Q0

Antitoxin

• AlphaFold DB: A0A3G5FKS5