Detailed information of TA system TA025667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazE(antitoxin)
• Location: 1018091..1018691
• Replicon: chromosome
• Accession: NC_016052
• Organism: Tetragenococcus halophilus NBRC 12172

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: TEH_RS04980
• Protein ID: WP_014124413.1
• Coordinates: 1018443..1018691 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A2H6C111
• Locus tag: TEH_RS04975
• Protein ID: WP_014124412.1
• Coordinates: 1018091..1018354 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TEH_RS04950 (TEH_10190)	1013357..1014358	+	1002	WP_014124406.1	NADP-dependent oxidoreductase	-
TEH_RS04955 (TEH_10200)	1014376..1015050	+	675	WP_014124407.1	type 1 glutamine amidotransferase domain-containing protein	-
TEH_RS13550 (TEH_10210)	1015466..1015741	+	276	WP_014124408.1	GyrI-like domain-containing protein	-
TEH_RS13555 (TEH_10220)	1015767..1015928	+	162	WP_014124409.1	hypothetical protein	-
TEH_RS04965 (TEH_10230)	1016081..1016962	+	882	WP_103083364.1	zinc-dependent alcohol dehydrogenase family protein	-
TEH_RS04970 (TEH_10240)	1017300..1018007	+	708	WP_014124411.1	hydroxyacylglutathione hydrolase	-
TEH_RS04975 (TEH_10250)	1018091..1018354	+	264	WP_014124412.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TEH_RS04980 (TEH_10260)	1018443..1018691	+	249	WP_014124413.1	hypothetical protein	Toxin
TEH_RS04985 (TEH_10270)	1018936..1019529	-	594	WP_014124414.1	DJ-1/PfpI family protein	-
TEH_RS13335 (TEH_10280)	1019726..1019962	+	237	WP_124175402.1	LD-carboxypeptidase	-
TEH_RS04990 (TEH_10290)	1020357..1021100	-	744	Protein_994	cyclase family protein	-
TEH_RS04995 (TEH_10300)	1021190..1021780	-	591	WP_014124417.1	DJ-1/PfpI family protein	-
TEH_RS05005 (TEH_10310)	1022146..1022844	+	699	WP_014124418.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (12-53)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9187.72 Da
• Isoelectric Point: 8.4127

>T25667 WP_014124413.1 NC_016052:1018443-1018691 [Tetragenococcus halophilus NBRC 12172]
MVQQISNMTIVAPVSSTKRGFPMYYSLESTKVVYGKVLLDQTIALNLQARNVTKADIVDQVSKKELTEIIAIYKFLFSVD
GE

Nucleotide

• Length: 249 bp

>T25667 NC_016052:1018443-1018691 [Tetragenococcus halophilus NBRC 12172]
ATGGTGCAACAAATAAGTAATATGACAATTGTAGCCCCCGTTAGCTCAACAAAACGTGGTTTTCCCATGTATTATTCTTT
AGAAAGTACCAAAGTTGTTTATGGAAAAGTTTTATTGGACCAAACAATTGCATTAAACCTACAAGCAAGAAATGTAACTA
AAGCAGACATTGTGGATCAGGTTTCTAAAAAAGAATTAACTGAGATCATCGCTATTTATAAATTCTTATTTAGCGTTGAT
GGTGAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9970.23 Da
• Isoelectric Point: 6.4568

>AT25667 WP_014124412.1 NC_016052:1018091-1018354 [Tetragenococcus halophilus NBRC 12172]
MNNIKGQTRITQWGNSMIIRIPSYIVKQLGLEDNQALTVTTRNNSIVLTPIKKQPTNIHDLFADWQDDGQRDHELDWGKA
EGNALPW

Nucleotide

• Length: 264 bp

>AT25667 NC_016052:1018091-1018354 [Tetragenococcus halophilus NBRC 12172]
ATGAATAATATTAAAGGTCAAACTCGTATTACACAGTGGGGAAATTCGATGATTATTCGAATCCCAAGTTATATTGTAAA
ACAATTAGGTTTAGAAGATAATCAAGCTTTAACTGTAACTACTCGAAATAATTCAATCGTTTTAACACCTATAAAAAAAC
AGCCGACTAATATTCATGACCTTTTTGCGGACTGGCAAGATGACGGTCAACGTGATCATGAGTTAGATTGGGGGAAAGCA
GAAGGTAACGCGTTGCCTTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2H6C111