Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 511936..512572 Replicon chromosome
Accession NZ_CP103784
Organism Bacillus subtilis strain RO-NN-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NYR91_RS02605 Protein ID WP_003156187.1
Coordinates 512222..512572 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NYR91_RS02600 Protein ID WP_003225183.1
Coordinates 511936..512217 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYR91_RS02580 (NYR91_02580) 508296..508895 - 600 WP_014475873.1 rhomboid family intramembrane serine protease -
NYR91_RS02585 (NYR91_02585) 508990..509355 + 366 WP_014475874.1 holo-ACP synthase -
NYR91_RS02590 (NYR91_02590) 509521..510537 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
NYR91_RS02595 (NYR91_02595) 510651..511820 + 1170 WP_014475875.1 alanine racemase -
NYR91_RS02600 (NYR91_02600) 511936..512217 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NYR91_RS02605 (NYR91_02605) 512222..512572 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NYR91_RS02610 (NYR91_02610) 512689..513513 + 825 WP_080009756.1 RsbT co-antagonist protein RsbRA -
NYR91_RS02615 (NYR91_02615) 513518..513883 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NYR91_RS02620 (NYR91_02620) 513887..514288 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
NYR91_RS02625 (NYR91_02625) 514300..515307 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
NYR91_RS02630 (NYR91_02630) 515376..515705 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
NYR91_RS02635 (NYR91_02635) 515702..516184 + 483 WP_014475878.1 anti-sigma B factor RsbW -
NYR91_RS02640 (NYR91_02640) 516150..516938 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NYR91_RS02645 (NYR91_02645) 516938..517537 + 600 WP_014475879.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T256664 WP_003156187.1 NZ_CP103784:512222-512572 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT256664 WP_003225183.1 NZ_CP103784:511936-512217 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References