Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 479684..480321 Replicon chromosome
Accession NZ_CP103782
Organism Bacillus velezensis strain DA4

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NYR48_RS02450 Protein ID WP_003156187.1
Coordinates 479971..480321 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NYR48_RS02445 Protein ID WP_003156188.1
Coordinates 479684..479965 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYR48_RS02425 476049..476648 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NYR48_RS02430 476741..477106 + 366 WP_007609580.1 holo-ACP synthase -
NYR48_RS02435 477271..478278 + 1008 WP_259425004.1 outer membrane lipoprotein carrier protein LolA -
NYR48_RS02440 478395..479564 + 1170 WP_259425005.1 alanine racemase -
NYR48_RS02445 479684..479965 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NYR48_RS02450 479971..480321 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NYR48_RS02455 480439..481260 + 822 WP_003156182.1 STAS domain-containing protein -
NYR48_RS02460 481265..481630 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NYR48_RS02465 481633..482034 + 402 WP_003156178.1 anti-sigma regulatory factor -
NYR48_RS02470 482046..483053 + 1008 WP_259425006.1 PP2C family protein-serine/threonine phosphatase -
NYR48_RS02475 483117..483446 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NYR48_RS02480 483443..483925 + 483 WP_007609591.1 anti-sigma B factor RsbW -
NYR48_RS02485 483891..484679 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NYR48_RS02490 484679..485281 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T256641 WP_003156187.1 NZ_CP103782:479971-480321 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT256641 WP_003156188.1 NZ_CP103782:479684-479965 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References