Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 460496..461060 Replicon chromosome
Accession NC_016052
Organism Tetragenococcus halophilus NBRC 12172

Toxin (Protein)


Gene name mazF Uniprot ID A0A2H6CPR8
Locus tag TEH_RS02190 Protein ID WP_014123850.1
Coordinates 460725..461060 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3G5FHJ2
Locus tag TEH_RS02185 Protein ID WP_041591760.1
Coordinates 460496..460732 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TEH_RS02165 (TEH_04490) 455583..456245 + 663 WP_014123845.1 HAD family phosphatase -
TEH_RS02170 (TEH_04500) 456266..457198 + 933 WP_014123846.1 tagatose-6-phosphate kinase -
TEH_RS02175 (TEH_04510) 457215..458186 + 972 WP_014123847.1 tagatose-bisphosphate aldolase -
TEH_RS02180 (TEH_04520) 458489..459595 + 1107 WP_014123848.1 IS4 family transposase -
TEH_RS12905 459886..459987 - 102 WP_069028690.1 putative holin-like toxin -
TEH_RS02185 (TEH_04530) 460496..460732 + 237 WP_041591760.1 hypothetical protein Antitoxin
TEH_RS02190 (TEH_04540) 460725..461060 + 336 WP_014123850.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
TEH_RS13285 461334..461623 + 290 Protein_438 hypothetical protein -
TEH_RS13495 461688..462055 + 368 Protein_439 zeta toxin family protein -
TEH_RS02200 (TEH_04570) 462324..463187 - 864 WP_014123853.1 glycine betaine ABC transporter substrate-binding protein -
TEH_RS02205 (TEH_04580) 463237..463797 - 561 WP_014123854.1 hypothetical protein -
TEH_RS02210 (TEH_04590) 464034..465509 + 1476 WP_197535348.1 betaine-aldehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12890.98 Da        Isoelectric Point: 8.9118

>T25664 WP_014123850.1 NC_016052:460725-461060 [Tetragenococcus halophilus NBRC 12172]
MTNHYTPEKGDIVWIDFDPSTGMEIQKRRPALVISRGNFNTATKFAVVCPITSTEIDLPTRYSLSENYKTKGQIVIHQMK
SLDFNKRKIDFIEKMNPVDLKKIEQIIEFII

Download         Length: 336 bp

>T25664 NC_016052:460725-461060 [Tetragenococcus halophilus NBRC 12172]
ATGACTAATCACTATACACCAGAAAAAGGAGACATAGTGTGGATCGACTTTGATCCTTCAACTGGAATGGAAATTCAAAA
GCGACGGCCAGCTCTGGTAATTTCGCGCGGAAATTTTAATACGGCGACAAAATTTGCGGTGGTATGTCCTATTACATCCA
CTGAAATAGATTTACCAACTAGATATAGTCTTTCAGAAAATTATAAAACAAAAGGACAAATAGTCATCCATCAAATGAAA
TCTTTAGATTTCAATAAACGCAAAATTGACTTTATTGAGAAAATGAACCCTGTGGACTTGAAGAAAATAGAACAGATCAT
TGAGTTCATTATTTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8506.60 Da        Isoelectric Point: 4.0338

>AT25664 WP_041591760.1 NC_016052:460496-460732 [Tetragenococcus halophilus NBRC 12172]
MLKTKTRAQGRSIVVTLPAEDGATVFPGKEYLVSYGNDGTIVLIPKIEDPFANVTEGAFYESDVWEDMPAVGKEVLDD

Download         Length: 237 bp

>AT25664 NC_016052:460496-460732 [Tetragenococcus halophilus NBRC 12172]
ATGCTAAAGACAAAAACAAGGGCACAAGGTAGATCTATCGTGGTTACTTTACCAGCTGAGGATGGGGCAACAGTTTTTCC
TGGGAAAGAATATCTTGTTTCATACGGAAATGATGGAACTATTGTTTTGATACCTAAAATTGAAGATCCGTTTGCTAATG
TTACAGAAGGAGCTTTTTACGAATCGGATGTATGGGAAGATATGCCAGCAGTTGGTAAAGAGGTGCTGGATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H6CPR8

Antitoxin

Source ID Structure
AlphaFold DB A0A3G5FHJ2

References