Detailed information of TA system TA025662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 936120..936745
• Replicon: chromosome
• Accession: NC_016051
• Organism: Thermococcus sp. AM4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G2M283
• Locus tag: TAM4_RS05150
• Protein ID: WP_014122185.1
• Coordinates: 936335..936745 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7R1W4
• Locus tag: TAM4_RS05145
• Protein ID: WP_014122184.1
• Coordinates: 936120..936350 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TAM4_RS05115 (TAM4_1780)	931665..932156	+	492	WP_014122179.1	DUF402 domain-containing protein	-
TAM4_RS05120 (TAM4_1784)	932153..932761	-	609	WP_014122180.1	tRNA (pseudouridine(54)-N(1))-methyltransferase TrmY	-
TAM4_RS05125 (TAM4_1794)	932820..933101	+	282	WP_014122181.1	DUF167 domain-containing protein	-
TAM4_RS05130 (TAM4_1715)	933314..934633	+	1320	WP_014122182.1	ATP-binding protein	-
TAM4_RS05135	934617..934949	+	333	WP_048150028.1	hypothetical protein	-
TAM4_RS05140 (TAM4_1605)	934939..936021	+	1083	WP_048150031.1	hypothetical protein	-
TAM4_RS05145 (TAM4_1501)	936120..936350	+	231	WP_014122184.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TAM4_RS05150 (TAM4_2398)	936335..936745	+	411	WP_014122185.1	PIN domain-containing protein	Toxin
TAM4_RS05155 (TAM4_1537)	936746..937753	-	1008	WP_014122186.1	phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-
TAM4_RS05160 (TAM4_1733)	937867..938694	+	828	WP_014122187.1	translation initiation factor IF-2	-
TAM4_RS05165 (TAM4_1495)	938691..939899	-	1209	WP_014122188.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
TAM4_RS05170 (TAM4_1610)	939954..940712	-	759	WP_048150034.1	DUF835 domain-containing protein	-
TAM4_RS05175 (TAM4_1648)	940843..941331	-	489	WP_014122190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (5-48)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15594.77 Da
• Isoelectric Point: 4.4505

>T25662 WP_014122185.1 NC_016051:936335-936745 [Thermococcus sp. AM4]
MHGVIDTNVLIYDTFEDTEFHKRAEEVLESLDRWYVPAIVVQEYVWFFKNNGFSAEEALEALKGYTEDPRFKGLSEDPQL
IESALDLVVAEKLSLSHFNDAVILLHTLERGTLVTFDVKLRNLAKRKGVVVLPEEL

Nucleotide

• Length: 411 bp

>T25662 NC_016051:936335-936745 [Thermococcus sp. AM4]
ATGCATGGGGTCATAGACACGAACGTCCTCATCTACGACACCTTCGAGGACACGGAGTTCCACAAAAGGGCGGAGGAGGT
ACTTGAATCCCTCGACAGGTGGTACGTTCCGGCGATAGTCGTGCAGGAGTACGTGTGGTTCTTCAAAAACAACGGCTTTT
CGGCGGAAGAAGCGCTCGAGGCCCTCAAGGGGTACACAGAGGATCCAAGATTCAAGGGATTGAGTGAAGACCCTCAACTA
ATCGAAAGCGCTCTGGACTTAGTGGTAGCCGAAAAACTCTCGCTCTCACATTTCAACGACGCGGTAATTCTTCTTCACAC
CCTGGAGAGGGGAACCCTCGTTACCTTCGACGTCAAGCTGAGAAACCTGGCAAAAAGAAAAGGGGTTGTTGTTCTCCCAG
AGGAGCTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8860.39 Da
• Isoelectric Point: 9.8801

>AT25662 WP_014122184.1 NC_016051:936120-936350 [Thermococcus sp. AM4]
MSITKVTRNYQITIPAEIRKALGIREGELLEVNIEGDRIVLRRFKRERKRLKLGRNVTPEEIEKSIEEGMMECMGS

Nucleotide

• Length: 231 bp

>AT25662 NC_016051:936120-936350 [Thermococcus sp. AM4]
ATGAGCATCACGAAAGTAACCCGCAACTACCAGATAACAATCCCGGCAGAGATTAGAAAGGCCCTTGGCATAAGGGAGGG
AGAACTCCTTGAAGTCAACATCGAGGGAGACAGAATAGTCCTTCGGAGGTTCAAAAGGGAAAGGAAGCGCCTGAAACTCG
GCAGGAACGTAACCCCCGAGGAGATAGAGAAGTCCATTGAGGAGGGCATGATGGAATGCATGGGGTCATAG

Structures

Toxin

• AlphaFold DB: G2M283

Antitoxin

• AlphaFold DB: B7R1W4