Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489948..490585 Replicon chromosome
Accession NZ_CP103770
Organism Bacillus velezensis strain TH-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NYO93_RS02515 Protein ID WP_003156187.1
Coordinates 490235..490585 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NYO93_RS02510 Protein ID WP_003156188.1
Coordinates 489948..490229 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYO93_RS02490 (NYO93_02490) 486313..486912 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
NYO93_RS02495 (NYO93_02495) 487005..487370 + 366 WP_007609580.1 holo-ACP synthase -
NYO93_RS02500 (NYO93_02500) 487535..488542 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
NYO93_RS02505 (NYO93_02505) 488659..489828 + 1170 WP_032873001.1 alanine racemase -
NYO93_RS02510 (NYO93_02510) 489948..490229 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NYO93_RS02515 (NYO93_02515) 490235..490585 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NYO93_RS02520 (NYO93_02520) 490703..491524 + 822 WP_003156182.1 STAS domain-containing protein -
NYO93_RS02525 (NYO93_02525) 491529..491894 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NYO93_RS02530 (NYO93_02530) 491897..492298 + 402 WP_003156178.1 anti-sigma regulatory factor -
NYO93_RS02535 (NYO93_02535) 492310..493317 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
NYO93_RS02540 (NYO93_02540) 493381..493710 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NYO93_RS02545 (NYO93_02545) 493707..494189 + 483 WP_007609591.1 anti-sigma B factor RsbW -
NYO93_RS02550 (NYO93_02550) 494155..494943 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
NYO93_RS02555 (NYO93_02555) 494943..495545 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T256617 WP_003156187.1 NZ_CP103770:490235-490585 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT256617 WP_003156188.1 NZ_CP103770:489948-490229 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References