Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2642911..2643548 Replicon chromosome
Accession NZ_CP103769
Organism Bacillus velezensis strain ML61

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NYR93_RS12575 Protein ID WP_003156187.1
Coordinates 2642911..2643261 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NYR93_RS12580 Protein ID WP_003156188.1
Coordinates 2643267..2643548 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYR93_RS12535 (NYR93_12540) 2637951..2638553 - 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -
NYR93_RS12540 (NYR93_12545) 2638553..2639341 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NYR93_RS12545 (NYR93_12550) 2639307..2639789 - 483 WP_003156173.1 anti-sigma B factor RsbW -
NYR93_RS12550 (NYR93_12555) 2639786..2640115 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NYR93_RS12555 (NYR93_12560) 2640179..2641186 - 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
NYR93_RS12560 (NYR93_12565) 2641198..2641599 - 402 WP_003156178.1 anti-sigma regulatory factor -
NYR93_RS12565 (NYR93_12570) 2641602..2641967 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
NYR93_RS12570 (NYR93_12575) 2641972..2642793 - 822 WP_003156182.1 STAS domain-containing protein -
NYR93_RS12575 (NYR93_12580) 2642911..2643261 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NYR93_RS12580 (NYR93_12585) 2643267..2643548 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NYR93_RS12585 (NYR93_12590) 2643668..2644837 - 1170 WP_003156189.1 alanine racemase -
NYR93_RS12590 (NYR93_12595) 2644954..2645961 - 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
NYR93_RS12595 (NYR93_12600) 2646126..2646491 - 366 WP_003156192.1 holo-ACP synthase -
NYR93_RS12600 (NYR93_12605) 2646584..2647183 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T256616 WP_003156187.1 NZ_CP103769:c2643261-2642911 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT256616 WP_003156188.1 NZ_CP103769:c2643548-2643267 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References