256615

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1842064..1842981 Replicon chromosome
Accession NZ_CP103769
Organism Bacillus velezensis strain ML61

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag NYR93_RS08630 Protein ID WP_003154806.1
Coordinates 1842064..1842810 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag NYR93_RS08635 Protein ID WP_003154807.1
Coordinates 1842811..1842981 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYR93_RS08610 (NYR93_08610) 1837455..1838405 - 951 WP_014304860.1 ring-cleaving dioxygenase -
NYR93_RS08615 (NYR93_08615) 1838729..1840045 + 1317 WP_003154801.1 amino acid permease -
NYR93_RS08620 (NYR93_08620) 1840331..1840948 + 618 WP_003154804.1 DUF47 domain-containing protein -
NYR93_RS08625 (NYR93_08625) 1840961..1841959 + 999 WP_003154805.1 inorganic phosphate transporter -
NYR93_RS08630 (NYR93_08630) 1842064..1842810 + 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
NYR93_RS08635 (NYR93_08635) 1842811..1842981 + 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
NYR93_RS08640 (NYR93_08640) 1843078..1843203 + 126 WP_003154809.1 hypothetical protein -
NYR93_RS08645 (NYR93_08645) 1843239..1844117 - 879 WP_014304858.1 N-acetylmuramoyl-L-alanine amidase -
NYR93_RS08650 (NYR93_08650) 1844131..1844394 - 264 WP_003154813.1 phage holin -
NYR93_RS08655 (NYR93_08655) 1844408..1844671 - 264 WP_003154815.1 hemolysin XhlA family protein -
NYR93_RS08660 (NYR93_08660) 1844723..1845484 - 762 WP_046559521.1 hypothetical protein -
NYR93_RS08665 (NYR93_08665) 1845541..1845738 - 198 WP_003154819.1 XkdX family protein -
NYR93_RS08670 (NYR93_08670) 1845744..1846115 - 372 WP_014304856.1 XkdW family protein -
NYR93_RS08675 (NYR93_08675) 1846128..1847750 - 1623 WP_003154821.1 pyocin knob domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1833885..1876722 42837
- inside Prophage - - 1834148..1883649 49501


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T256615 WP_003154806.1 NZ_CP103769:1842064-1842810 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT256615 WP_003154807.1 NZ_CP103769:1842811-1842981 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References