Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 113837..114480 Replicon plasmid pMB9880_1
Accession NZ_CP103763
Organism Escherichia coli strain 5283

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag M5T11_RS25495 Protein ID WP_001044768.1
Coordinates 114064..114480 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag M5T11_RS25490 Protein ID WP_001261287.1
Coordinates 113837..114067 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T11_RS25480 (109015) 109015..110148 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
M5T11_RS25485 (110411) 110411..113530 - 3120 WP_023909028.1 hypothetical protein -
M5T11_RS25490 (113837) 113837..114067 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M5T11_RS25495 (114064) 114064..114480 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
M5T11_RS25500 (114642) 114642..116780 - 2139 WP_000350635.1 AAA family ATPase -
M5T11_RS25505 (117245) 117245..118240 + 996 WP_000246636.1 hypothetical protein -
M5T11_RS25510 (118283) 118283..119176 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(B) / dfrA17 / aadA5 / qacE / sul1 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IId / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 - 1..128773 128773
- flank IS/Tn - - 119313..119816 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T256610 WP_001044768.1 NZ_CP103763:114064-114480 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT256610 WP_001261287.1 NZ_CP103763:113837-114067 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References