Detailed information of TA system TA256597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3933222..3934911
• Replicon: chromosome
• Accession: NZ_CP103762
• Organism: Escherichia coli strain 5283

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: M5T11_RS19105
• Protein ID: WP_001521912.1
• Coordinates: 3933222..3934142 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: M5T11_RS19110
• Protein ID: WP_001521911.1
• Coordinates: 3934129..3934911 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5T11_RS19090 (3929312)	3929312..3930439	-	1128	WP_001521913.1	MFS transporter	-
M5T11_RS19095 (3930581)	3930581..3931639	-	1059	WP_122452191.1	alpha/beta hydrolase	-
M5T11_RS19100 (3931904)	3931904..3932788	+	885	WP_001119037.1	LysR family transcriptional regulator	-
M5T11_RS19105 (3933222)	3933222..3934142	-	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5T11_RS19110 (3934129)	3934129..3934911	-	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M5T11_RS19115 (3935227)	3935227..3938436	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
M5T11_RS19120 (3938783)	3938783..3939253	+	471	WP_000201185.1	hypothetical protein	-
M5T11_RS19125 (3939250)	3939250..3939352	+	103	Protein_3745	helix-turn-helix domain-containing protein	-
M5T11_RS19130 (3939444)	3939444..3939602	+	159	Protein_3746	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T256597 WP_001521912.1 NZ_CP103762:c3934142-3933222 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT256597 WP_001521911.1 NZ_CP103762:c3934911-3934129 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A829L165