Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3056149..3056933 Replicon chromosome
Accession NZ_CP103762
Organism Escherichia coli strain 5283

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag M5T11_RS15095 Protein ID WP_000613626.1
Coordinates 3056439..3056933 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag M5T11_RS15090 Protein ID WP_001110447.1
Coordinates 3056149..3056442 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T11_RS15080 (3051299) 3051299..3052258 - 960 WP_000846343.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
M5T11_RS15085 (3052831) 3052831..3056016 + 3186 WP_001522867.1 ribonuclease E -
M5T11_RS15090 (3056149) 3056149..3056442 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
M5T11_RS15095 (3056439) 3056439..3056933 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
M5T11_RS15100 (3057028) 3057028..3057981 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
M5T11_RS15105 (3057993) 3057993..3059636 - 1644 WP_001522865.1 flagellar hook-associated protein FlgK -
M5T11_RS15110 (3059702) 3059702..3060643 - 942 WP_001522863.1 flagellar assembly peptidoglycan hydrolase FlgJ -
M5T11_RS15115 (3060643) 3060643..3061740 - 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T256594 WP_000613626.1 NZ_CP103762:3056439-3056933 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT256594 WP_001110447.1 NZ_CP103762:3056149-3056442 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References