Detailed information of TA system TA025658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 635414..636050
• Replicon: chromosome
• Accession: NC_016047
• Organism: Bacillus subtilis subsp. spizizenii TU-B-10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: GYO_RS24940
• Protein ID: WP_003156187.1
• Coordinates: 635700..636050 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: GYO_RS24935
• Protein ID: WP_003225183.1
• Coordinates: 635414..635695 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYO_RS24915	631774..632373	-	600	WP_003225175.1	rhomboid family intramembrane serine protease	-
GYO_RS24920	632468..632833	+	366	WP_014112730.1	holo-ACP synthase	-
GYO_RS24925	632998..634014	+	1017	WP_072181690.1	outer membrane lipoprotein carrier protein LolA	-
GYO_RS24930	634129..635298	+	1170	WP_014112731.1	alanine racemase	-
GYO_RS24935	635414..635695	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
GYO_RS24940	635700..636050	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GYO_RS24945	636167..636991	+	825	WP_003240350.1	RsbT co-antagonist protein RsbRA	-
GYO_RS24950	636996..637361	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
GYO_RS24955	637365..637766	+	402	WP_014112733.1	serine/threonine-protein kinase RsbT	-
GYO_RS24960	637778..638785	+	1008	WP_014112734.1	phosphoserine phosphatase RsbU	-
GYO_RS24965	638847..639176	+	330	WP_014112735.1	anti-sigma factor antagonist RsbV	-
GYO_RS24970	639173..639655	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
GYO_RS24975	639621..640409	+	789	WP_003240361.1	RNA polymerase sigma factor SigB	-
GYO_RS24980	640409..641008	+	600	WP_014112736.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T25658 WP_003156187.1 NC_016047:635700-636050 [Bacillus subtilis subsp. spizizenii TU-B-10]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T25658 NC_016047:635700-636050 [Bacillus subtilis subsp. spizizenii TU-B-10]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT25658 WP_003225183.1 NC_016047:635414-635695 [Bacillus subtilis subsp. spizizenii TU-B-10]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT25658 NC_016047:635414-635695 [Bacillus subtilis subsp. spizizenii TU-B-10]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32