Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4017492..4018068 Replicon chromosome
Accession NZ_CP103758
Organism Escherichia coli strain p10B

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NYO15_RS19660 Protein ID WP_001297643.1
Coordinates 4017781..4018068 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NYO15_RS19655 Protein ID WP_000063156.1
Coordinates 4017492..4017794 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYO15_RS19640 (4014088) 4014088..4016238 + 2151 WP_001446922.1 pyruvate/proton symporter BtsT -
NYO15_RS19645 (4016288) 4016288..4016491 + 204 WP_000467859.1 YbdD/YjiX family protein -
NYO15_RS19650 (4016502) 4016502..4017458 + 957 WP_001297640.1 GTPase -
NYO15_RS19655 (4017492) 4017492..4017794 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NYO15_RS19660 (4017781) 4017781..4018068 - 288 WP_001297643.1 BrnT family toxin Toxin
NYO15_RS19665 (4018267) 4018267..4018964 - 698 Protein_3841 IS1 family transposase -
NYO15_RS19670 (4019021) 4019021..4019116 + 96 Protein_3842 dienelactone hydrolase family protein -
NYO15_RS19675 (4019262) 4019262..4020494 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
NYO15_RS19680 (4020535) 4020535..4021815 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T256573 WP_001297643.1 NZ_CP103758:c4018068-4017781 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT256573 WP_000063156.1 NZ_CP103758:c4017794-4017492 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References