Detailed information of TA system TA025657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1350022..1351769
• Replicon: chromosome
• Accession: NC_016041
• Organism: Glaciecola nitratireducens FR1064

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: G4QKY3
• Locus tag: GNIT_RS05930
• Protein ID: WP_014108247.1
• Coordinates: 1350022..1350945 (-)
• Length: 308 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: G4QKY4
• Locus tag: GNIT_RS05935
• Protein ID: WP_014108248.1
• Coordinates: 1350978..1351769 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNIT_RS05915	1346032..1347090	+	1059	WP_014108244.1	quinolinate synthase NadA	-
GNIT_RS05925	1347365..1349527	+	2163	WP_041246314.1	tyrosine-type recombinase/integrase	-
GNIT_RS05930	1350022..1350945	-	924	WP_014108247.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GNIT_RS05935	1350978..1351769	-	792	WP_014108248.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GNIT_RS05940	1352437..1353843	-	1407	WP_014108250.1	sel1 repeat family protein	-
GNIT_RS05945	1354419..1355603	-	1185	WP_014108251.1	IS91 family transposase	-
GNIT_RS05950	1355614..1356486	-	873	WP_014108252.1	tyrosine-type recombinase/integrase	-
GNIT_RS18160	1356601..1356762	-	162	WP_158307650.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1347365..1376890	29525

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-272)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-97)

Sequences

Toxin

Protein

• Length: 308 a.a.
• Molecular weight: 34900.32 Da
• Isoelectric Point: 6.3987

>T25657 WP_014108247.1 NC_016041:c1350945-1350022 [Glaciecola nitratireducens FR1064]
MEKNSIYYKQVKMLVGVLPIVAKEKDFALKGGTAINLFVRDFPRLSVDIDLAYLPLEDRNTALANARLALNRIADAINAS
MKARATLQDNKPDELRIIVSDASATIKVEVSPVARGTLYEPTTMMVHDKVEDEFGYAEIQVVSMPDLYGGKLCAAMDRQH
PRDLFDVKLLLENEGISRKILIGFLTYVLSHPRPIHEVMQPNWKDLSAVFRSEFEGMTFVAVTEDELKQTRIKMLDALKH
HFDNRDAEFLLSFKRGQPNWTLFDEPSIADLPAVKWKLRNIAKLMQDAEKHDAQVTNLEKSLTNWIS

Nucleotide

• Length: 924 bp

>T25657 NC_016041:c1350945-1350022 [Glaciecola nitratireducens FR1064]
ATGGAAAAAAACAGTATTTATTACAAACAAGTGAAAATGCTCGTAGGCGTTTTACCTATTGTAGCAAAGGAAAAAGACTT
CGCTTTAAAAGGCGGTACTGCAATAAATCTCTTTGTTAGAGACTTCCCGAGATTGTCCGTTGATATCGACCTAGCCTATT
TGCCCCTAGAGGATCGCAATACAGCCCTTGCTAATGCGAGATTAGCACTCAACCGTATAGCAGACGCAATAAATGCCAGT
ATGAAAGCGAGAGCAACATTACAGGACAATAAGCCTGATGAACTGCGCATCATAGTAAGCGATGCGTCTGCCACCATTAA
AGTAGAGGTATCACCCGTTGCCAGAGGCACGCTCTATGAGCCTACAACAATGATGGTGCACGACAAAGTGGAAGACGAAT
TTGGATATGCTGAAATACAAGTAGTCAGCATGCCTGATTTATATGGAGGTAAGCTTTGTGCGGCAATGGACAGGCAGCAC
CCTAGAGACTTATTCGATGTAAAACTACTACTGGAGAACGAAGGCATATCTCGCAAGATACTAATTGGCTTTTTAACCTA
TGTTTTGAGCCATCCACGGCCTATTCATGAAGTAATGCAGCCAAATTGGAAAGACTTATCAGCTGTATTTCGTTCCGAGT
TTGAAGGGATGACCTTTGTCGCAGTCACCGAAGATGAACTGAAGCAGACGCGTATCAAGATGTTAGATGCTTTGAAACAT
CATTTTGATAATAGAGACGCAGAATTTCTTCTATCATTCAAGCGTGGTCAGCCTAATTGGACTCTCTTTGATGAGCCCTC
TATTGCCGACTTACCCGCAGTGAAGTGGAAGCTCAGAAATATTGCTAAACTAATGCAAGACGCTGAAAAGCACGATGCAC
AGGTAACAAACCTAGAGAAATCGCTCACTAATTGGATAAGTTGA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 29739.11 Da
• Isoelectric Point: 9.9624

>AT25657 WP_014108248.1 NC_016041:c1351769-1350978 [Glaciecola nitratireducens FR1064]
MNSKLNLLVNEFSPGSVVLQQWLGQNGISHSLAQKYVQNGWLERLANGVFYRPQKDPKVLPTWATVLSALMYQTDIKVHL
AGLSSLQQQGLSHYLSLNSTNTTNQLKNKVWIGTDGKQTLPKWFREFCQVEWIYCKNKVDNQHVKACIPITVNGKALLAS
SAELAAFEVVSAIGRDISFEHVAELFQGLVNLSPRKVQTILASSKAIQANRVFLFLGHYYEHQWVKRIDESVVNLGAGKR
QVVANGHYDRQYKITVPSDFRFH

Nucleotide

• Length: 792 bp

>AT25657 NC_016041:c1351769-1350978 [Glaciecola nitratireducens FR1064]
ATGAATTCAAAGTTAAACTTACTCGTTAATGAATTCTCACCTGGTTCTGTTGTTTTACAACAATGGTTAGGGCAAAACGG
CATTAGCCACTCATTAGCGCAGAAATACGTCCAAAATGGCTGGTTAGAACGATTAGCTAACGGTGTTTTTTATCGTCCTC
AAAAAGACCCAAAAGTGCTACCAACATGGGCGACCGTGCTGAGTGCGCTAATGTACCAAACTGATATAAAAGTCCACCTT
GCGGGGCTATCTAGCTTGCAACAACAGGGACTCTCGCACTATTTATCGCTCAACTCGACTAACACTACAAATCAATTAAA
AAATAAGGTGTGGATAGGCACCGATGGCAAACAGACTTTGCCAAAATGGTTCAGAGAATTTTGCCAAGTAGAATGGATCT
ACTGTAAAAATAAAGTCGACAATCAGCATGTTAAAGCTTGCATACCAATAACCGTAAATGGCAAAGCACTATTAGCCAGC
TCGGCAGAATTGGCTGCATTTGAAGTTGTTTCAGCTATAGGCAGAGACATCAGTTTCGAACACGTCGCAGAGCTCTTCCA
GGGTTTGGTAAACCTCAGTCCGAGAAAAGTGCAAACAATATTAGCAAGCAGTAAGGCCATACAAGCCAACAGAGTCTTTT
TATTTTTAGGCCATTATTATGAGCATCAATGGGTAAAACGCATAGATGAATCAGTAGTCAATTTAGGCGCAGGCAAAAGG
CAAGTCGTTGCCAATGGTCATTATGACCGACAATACAAAATCACGGTACCGTCCGATTTTAGGTTCCATTAA

Structures

Toxin

• AlphaFold DB: G4QKY3

Antitoxin

• AlphaFold DB: G4QKY4