Detailed information of TA system TA025655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2572815..2573450
• Replicon: chromosome
• Accession: NC_016027
• Organism: Gluconacetobacter medellinensis NBRC 3288

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GLX_RS12105
• Protein ID: WP_014106242.1
• Coordinates: 2573040..2573450 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G2I1D7
• Locus tag: GLX_RS12100
• Protein ID: WP_010511771.1
• Coordinates: 2572815..2573039 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLX_RS17480	2568002..2568889	-	888	WP_014106237.1	LysR family transcriptional regulator	-
GLX_RS12085	2569085..2570194	+	1110	WP_014106238.1	NADH:flavin oxidoreductase/NADH oxidase	-
GLX_RS17485	2570388..2571044	+	657	WP_148268597.1	alpha-ketoglutarate-dependent dioxygenase AlkB	-
GLX_RS12090	2571094..2571990	+	897	WP_014106240.1	NAD-dependent epimerase/dehydratase family protein	-
GLX_RS12095	2572216..2572542	-	327	WP_050856127.1	hypothetical protein	-
GLX_RS12100	2572815..2573039	+	225	WP_010511771.1	hypothetical protein	Antitoxin
GLX_RS12105	2573040..2573450	+	411	WP_014106242.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GLX_RS18490	2573607..2573786	+	180	WP_158309237.1	hypothetical protein	-
GLX_RS17490	2573750..2573881	+	132	Protein_2415	IS3 family transposase	-
GLX_RS12115	2574258..2575193	+	936	WP_014106243.1	LysR family transcriptional regulator	-
GLX_RS12120	2575249..2577588	+	2340	WP_014106244.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2457743..2601364	143621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (9-55)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15558.79 Da
• Isoelectric Point: 6.4680

>T25655 WP_014106242.1 NC_016027:2573040-2573450 [Komagataeibacter medellinensis NBRC 3288]
MLRYLLDTNLCIRVLRDRPQGLRPRFNSCAEELCISDVVLYELLYGAERSSDPVRTRREVERFAARLAVLPFDSEAAAHT
ADIRAELERNGRIIGPYDLMIAGHARSRGLIVVTGNLREFQRVEGLRTEDWLTDTV

Nucleotide

• Length: 411 bp

>T25655 NC_016027:2573040-2573450 [Komagataeibacter medellinensis NBRC 3288]
ATGCTGCGTTATCTGCTGGATACGAACCTCTGTATCCGGGTATTGCGGGATCGACCACAAGGGCTGCGTCCAAGGTTCAA
CAGCTGTGCGGAGGAACTCTGCATCTCGGACGTGGTTCTGTATGAGCTGCTTTATGGCGCAGAACGCTCGTCCGATCCCG
TGCGCACAAGGCGTGAAGTCGAGCGCTTCGCGGCAAGGCTGGCGGTCCTGCCATTCGACAGTGAAGCAGCCGCCCATACG
GCAGACATTCGGGCCGAACTTGAACGGAATGGCCGTATCATAGGTCCGTATGATCTCATGATTGCCGGGCATGCCCGGAG
CAGAGGGCTGATTGTTGTGACCGGCAATCTGCGGGAATTTCAGCGGGTGGAGGGTCTTCGAACCGAGGACTGGCTGACTG
ACACGGTCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8217.39 Da
• Isoelectric Point: 4.9816

>AT25655 WP_010511771.1 NC_016027:2572815-2573039 [Komagataeibacter medellinensis NBRC 3288]
MLTRTTLFLSNRSQAVRLPKMVAFGEQVRDVVIVSEGSRRIIAPADAAWDDFFAAPGVDLGERNQPAMQEREAL

Nucleotide

• Length: 225 bp

>AT25655 NC_016027:2572815-2573039 [Komagataeibacter medellinensis NBRC 3288]
ATGCTGACCCGTACGACACTCTTTCTATCCAACCGCTCGCAGGCAGTGCGCCTGCCGAAGATGGTCGCCTTCGGGGAGCA
GGTGCGTGACGTCGTCATTGTGTCCGAAGGTTCACGACGCATCATCGCCCCCGCTGACGCAGCGTGGGATGATTTCTTTG
CGGCTCCCGGCGTGGATCTGGGGGAGCGGAACCAGCCGGCCATGCAGGAACGTGAGGCGCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A094ZE42