Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4365038..4365614 Replicon chromosome
Accession NZ_CP103755
Organism Escherichia coli strain p11B

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag NYO14_RS21775 Protein ID WP_001295743.1
Coordinates 4365327..4365614 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag NYO14_RS21770 Protein ID WP_000063148.1
Coordinates 4365038..4365340 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYO14_RS21755 (4361634) 4361634..4363784 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NYO14_RS21760 (4363834) 4363834..4364037 + 204 WP_000467859.1 YbdD/YjiX family protein -
NYO14_RS21765 (4364048) 4364048..4365004 + 957 WP_001295745.1 GTPase -
NYO14_RS21770 (4365038) 4365038..4365340 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
NYO14_RS21775 (4365327) 4365327..4365614 - 288 WP_001295743.1 BrnT family toxin Toxin
NYO14_RS21780 (4365811) 4365811..4366977 + 1167 WP_000800831.1 restriction endonuclease -
NYO14_RS21785 (4367041) 4367041..4368660 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
NYO14_RS21790 (4368650) 4368650..4369954 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T256542 WP_001295743.1 NZ_CP103755:c4365614-4365327 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT256542 WP_000063148.1 NZ_CP103755:c4365340-4365038 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References