Detailed information of TA system TA256540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3986681..3988370
• Replicon: chromosome
• Accession: NZ_CP103755
• Organism: Escherichia coli strain p11B

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: NYO14_RS19950
• Protein ID: WP_000365807.1
• Coordinates: 3986681..3987601 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: NYO14_RS19955
• Protein ID: WP_001295795.1
• Coordinates: 3987588..3988370 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NYO14_RS19910 (3982154)	3982154..3982864	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
NYO14_RS19915 (3983171)	3983171..3983500	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
NYO14_RS19920 (3983512)	3983512..3983678	-	167	Protein_3914	tail fiber assembly protein	-
NYO14_RS19925 (3983749)	3983749..3984363	-	615	WP_001019923.1	YagU family protein	-
NYO14_RS19930 (3984578)	3984578..3984763	-	186	WP_000146240.1	hypothetical protein	-
NYO14_RS19935 (3984812)	3984812..3984970	+	159	Protein_3917	integrase	-
NYO14_RS19940 (3985197)	3985197..3985532	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
NYO14_RS19945 (3985740)	3985740..3986516	+	777	WP_000171155.1	site-specific integrase	-
NYO14_RS19950 (3986681)	3986681..3987601	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
NYO14_RS19955 (3987588)	3987588..3988370	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
NYO14_RS19960 (3988654)	3988654..3991752	+	3099	WP_000673070.1	type I restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3946543..3995433	48890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T256540 WP_000365807.1 NZ_CP103755:c3987601-3986681 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT256540 WP_001295795.1 NZ_CP103755:c3988370-3987588 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0